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PCR amplification of species specific sequences of 16S rDNA and 16S-23S rDNA intergenic spacer region for identification of Streptococcus phocae

Artículo de investigación publicado en Microbiological Research

15 de marzo de 2008

Streptococcus phocae, a bacterial pathogen of seals, could reliably be identified by PCR amplification using oligonucleotide primers designed according to species specific segments of the previously sequenced 16S rRNA gene and the 16S-23S rDNA intergenic spacer region of this species. The PCR mediated assay allowed an identification of S. phocae isolated from harbor and gray seals and from Atlantic salmons. No cross-reaction could be observed with 13 different other streptococcal species and subspecies and with Lactococcus garvieae strains investigated for control purposes




Hassan AA., Vossen A., Lammler C., Siebert U. y Fernandez-Garayzabal JF..




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PCR amplification of species specific sequences of 16S rDNA and 16S-23S rDNA intergenic spacer region for identification of Streptococcus phocae

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PCR amplification of species specific sequences of 16S rDNA and 16S-23S rDNA intergenic spacer region for identification of Streptococcus phocae


Participantes:

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Justus-Liebig-Universität GießenInstitut für Pharmakologie und Toxikologie. Justus-Liebig-Universität Gießen (JLU).

Justus-Liebig-Universität GießenInstitut für Tierärztliche Nahrungsmittelkunde (IfTN). Justus-Liebig-Universität Gießen (JLU).

Rhein–Sieg–Kreis.

Forschung-und Technologiezentrum Westküste. Christian-Albrechts-Universität zu Kiel (CAU).







FACTOR YEAR Q
2.054 2008

NLMID: 9437794

PMID: 16793248

ISSN: 0944-5013



TÍTULO: PCR amplification of species specific sequences of 16S rDNA and 16S-23S rDNA intergenic spacer region for identification of Streptococcus phocae


REVISTA: Microbiol. Res.


NUMERACIÓN: 163(2):132-5


AÑO: 2008


EDITORIAL: G. Fischer


AUTORES: Hassan AA., Vossen A., Lammler C., Siebert U. and Fernandez-Garayzabal JF..


PARTICIPANTES VISAVET


José Francisco Fernández-Garayzabal Fernández

DOI: https://doi.org/10.1016/j.micres.2006.01.017


CITA ESTA PUBLICACIÓN:

Hassan AA., Vossen A., Lammler C., Siebert U. y Fernandez-Garayzabal JF. PCR amplification of species specific sequences of 16S rDNA and 16S-23S rDNA intergenic spacer region for identification of Streptococcus phocae. Microbiological Research. 163(2):132-5. 2008. (A). ISSN: 0944-5013. DOI: 10.1016/j.micres.2006.01.017


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