PCR amplification of species specific sequences of 16S rDNA and 16S-23S rDNA intergenic spacer region for identification of Streptococcus phocae
Artículo de investigación publicado en Microbiological Research
15 de marzo de 2008
Streptococcus phocae, a bacterial pathogen of seals, could reliably be identified by PCR amplification using oligonucleotide primers designed according to species specific segments of the previously sequenced 16S rRNA gene and the 16S-23S rDNA intergenic spacer region of this species. The PCR mediated assay allowed an identification of S. phocae isolated from harbor and gray seals and from Atlantic salmons. No cross-reaction could be observed with 13 different other streptococcal species and subspecies and with Lactococcus garvieae strains investigated for control purposes
Hassan AA., Vossen A., Lammler C., Siebert U. y Fernandez-Garayzabal JF..
 | Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM). |
| Servicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM). |
 | Institut für Pharmakologie und Toxikologie. Justus-Liebig-Universität Gießen (JLU). |
| Institut für Tierärztliche Nahrungsmittelkunde (IfTN). Justus-Liebig-Universität Gießen (JLU). |
| Rhein–Sieg–Kreis. | |
| Forschung-und Technologiezentrum Westküste. Christian-Albrechts-Universität zu Kiel (CAU). | |
