Pseudomonas composti sp. nov., isolated from compost samples
International Journal of Systematic and Evolutionary Microbiology publica este artículo de investigación
1 de diciembre de 2011
Two unusual gram-negative, catalase- and oxidase-positive, rod-shaped organisms (C2(T) and C5) isolated from compost samples were characterized by phenotypic and molecular genetic methods. Comparative 16S rRNA gene sequencing studies demonstrated that both isolates were members of the genus Pseudomonas and located in the Pseudomonas aeruginosa group. Pseudomonas cuatrocienegasensis and Pseudomonas borbori were the most closely related species with a 16S rRNA gene sequence similarities of 97.9% and 97.8%, respectively. However, phylogenetic analyses based on rpoD sequences revealed that both isolates can be discriminated from Pseudomonas species that exhibited >97% similarity in 16S rRNA sequence and species belonging to the Pseudomonas aeruginosa group. The DNA G+C content of the type strain is 61.5 mol%. The major fatty acids of the strain C2(T) were C(18:1)ω7c/12t/9t, C(16:0), C(12:0), and a combined feature of C(16:1)ω7c and iso-C(15:0) 2-0H which supports its affiliation to the genus Pseudomonas. Moreover, the new bacterium was distinguished from its closet phylogenetically related species of Pseudomonas by DNA-DNA hybridization studies and biochemical tests. Based on both phenotypic and phylogenetic findings, it is proposed that the unknown Pseudomonas isolated from compost would be classified as Pseudomonas composti sp. nov. The type strain of Pseudomonas composti is C2(T) (=CECT 7516(T) =CCUG 59231(T))
Gibello A., Vela AI., Martin M., Mengs G., Alonso PZ., Garbi C. y Fernandez-Garayzabal JF..
 | Servicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM). |
| Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM). |
| Departamento de Bioquímica y Biología Molecular IV. Facultad de Veterinaria. Universidad Complutense (UCM). |
| Natural Biotec S.L. | |
