Flavobacterium tructae sp. nov. and Flavobacterium piscis sp. nov. isolated from farmed rainbow trout (Oncorhynchus mykiss)
Artículo de investigación publicado en International Journal of Systematic and Evolutionary Microbiology
1 de febrero de 2014
Four Gram-staining-negative, catalase- and oxidase-positive, pale-orange pigmented bacterial strains (435-08T, 47B-3-09, 412R-09T and 60B-3-09) were isolated from diseased rainbow trout. Analysis of their 16S rRNA gene sequences showed their adscription to the genus Flavobacterium. Strains formed two phylogenetic groups represented by strains 435-08T and 47B-3-09 (group A), and strains 412R-09T and 60B-3-09 (group B) displaying 16S rRNA sequence similarities greater than 99.8-99.9% within their respective groups. Strain 435-08T exhibited the highest levels of similarity with Flavobacterium aquidurense WB-1.1.56T (98.6% sequence similarity) and strain 412R-09T with Flavobacterium frigidimaris KUC-1T and Flavobacterium aquidurense WB-1.1.56T (98.9% and 98.6% sequence similarity, respectively). DNA-DNA hybridization studies showed low levels of relatedness between strain 435-08T and strain 412R-09T and between both strains and their most closely related Flavobacterium species. The genomic DNA G+C contents of strains 435-08T and 412R-09T were 36.2 and 34.3 mol%, respectively. The predominant respiratory quinone of both strains was MK-6 and the major fatty acids were iso-C15:0, C16:1 ω7c and C15:0. Both groups of strains were distinguished between them and from related Flavobacterium species by a number of phenotypic properties. Phylogenetic, genotypic and phenotypic evidences indicate that strains of group A and B represent two new species of the genus Flavobacterium, for which the names Flavobacterium tructae sp. nov (type strain 435-08T =CECT 7791T = CCUG 60100T) and Flavobacterium piscis sp. nov. (type strain 412R-09T =CECT 7911T = CCUG 60099T), are proposed
Zamora L., Vela AI., Sanchez-Porro C., Palacios MA., Moore ERB., Dominguez L., Ventosa A. y Fernandez-Garayzabal JF..
Servicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM). | |
Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM). | |
Piscifactoría Zorita Illana, S.A. (PISZOLLA). | |
Facultad de Farmacia. Universidad de Sevilla (US). | |
Sahlgrenska Academy. University of Gothenburg. | |