Investigación publicada en Veterinary Research

2 de mayo de 2019

Lactococcus garvieae is a significant pathogen in aquaculture with a potential zoonotic risk. To begin to characterize the late immune response of trout to lactococcosis, we selected infected individuals showing clinical signs of lactococcosis. At the time lactococcosis clinical signs appeared, infection by L. garvieae induced a robust inflammatory response in the spleen of rainbow trout, which correlated with abundant granulomatous lesions. The response in kidney goes in parallel with that of spleen, and most of the gene regulations are similar in both organs. A correlation existed between the early inflammatory granulomas in spleen (containing macrophages with internalized L. garvieae) and up-regulated gene sets, which defined the presence of macrophages and neutrophils. This is the first analysis of the immune transcriptome of rainbow trout following L. garvieae infection during the initiation of adaptive immune mechanisms and shows a transcriptome induction of antibody response by both IgM (+) and IgT (+) spleen B cells to respond to systemic infection. These results increase our understanding of lactococcosis and pave the way for future research to improve control measures of lactococcosis on fish farms

Castro R., Coll J., Blanco MDM., Rodriguez-Bertos A., Jouneau L., Fernandez-Garayzabal JF. y Gibello A.

Spleen and head kidney differential gene expression patterns in trout infected with Lactococcus garvieae correlate with spleen granulomas

Spleen and head kidney differential gene expression patterns in trout infected with Lactococcus garvieae correlate with spleen granulomas

	Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).
	Institut National de la Recherche Agronomique (INRA).
	Virologie et Immunologie Moléculaires. Université Paris-Saclay.
	Departamento de Biotecnología. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA).
	Departamento de Medicina y Cirugía Animal. Facultad de Veterinaria. Universidad Complutense (UCM).
	Servicio de Identificación y Caracterización Microbiana (ICM). Servicio de Patología y Veterinaria Forense (SAP). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).