Comunicación presentada en AHVLA International Conference 2011

15 de septiembre de 2011

Zamora L., Fernandez-Garayzabal JF., Palacio MA., Sanchez-Porro C., Moore E., Dominguez L., Ventosa A. y Vela AI.

Chryseobacterium is still a rapidly evolving genus with many new additions having been made over the last years. Several of these species, such as Chryseobacterium balustinum, Chryseobacterium scophtalmum, Chryseobacterium joostei and more recently, Chryseobacterium piscicola, Chryseobacterium arothri and Chryseobacterium chaponense have been associated with infections in fish. In fact, Chryseobacterium are considered potentially emerging fish pathogens. During the routine microbiological diagnosis from different clinical specimens of rainbow trout (Oncorhynchus mykiss) submitted to the VISAVET Health Surveillance Centre of the Universidad Complutense (Madrid, Spain), several Gram-negative, catalase and oxidase-positive, rod-shaped bacteria were isolated and their taxonomic status determined using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that the trout isolates belong to the genus Chryseobacterium and formed three different lineages clustering within a subgroup of 16 species of Chryseobacterim that had 16S rRNA gene sequence similarities >=97.0%. DNA–DNA hybridization values between the trout isolates and the type strain of species of Chryseobacterium phylogenetically closely related was always <70%, confirming that they belong to a distinct genomic species. Results of the respiratory quinone and fatty acids analysis were in accordance with those of members of the genus Cryseobacterium. The novel isolates were distinguished from phylogenetic related Chryseobacterium species by physiological and biochemical tests. The genetic and phenotypic properties of the trout isolates suggest their classification as representatives of novel species of the genus Chryseobacterium, for which the name Chryseobacterium oncorhynchi sp. nov.; Chryseobacterium piscis sp. nov., and Chryseobacterium tructae sp. nov., are proposed

	Servicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).
	Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Enlace a AHVLA International Conference 2011