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The highly dynamic CRISPR1 system of Streptococcus agalactiae controls the diversity of its mobilome

Investigación publicada en Molecular Microbiology

1 de septiembre de 2012

Clustered regularly interspaced short palindromic repeats (CRISPR) confer immunity against mobile genetic elements (MGEs) in prokaryotes. Streptococcus agalactiae, a leading cause of neonatal infections contains in its genome two CRISPR/Cas systems. We show that type 1-C CRISPR2 is present in few strains but type 2-A CRISPR1 is ubiquitous. Comparative sequence analysis of the CRISPR1 spacer content of 351 S. agalactiae strains revealed that it is extremely diverse due to the acquisition of new spacers, spacer duplications and spacer deletions that witness the dynamics of this system. The spacer content profile mirrors the S. agalactiae population structure. Transfer of a conjugative transposon targeted by CRISPR1 selected for spacer rearrangements, suggesting that deletions and duplications pre-exist in the population. The comparison of protospacers located within MGE or the core genome and protospacer-associated motif-shuffling demonstrated that the GG motif is sufficient to discriminate self and non-self and for spacer selection and integration. Strikingly more than 40% of the 949 different CRISPR1 spacers identified target MGEs found in S. agalactiae genomes. We thus propose that the S. agalactiae type II-A CRISPR1/Cas system modulates the cohabitation of the species with its mobilome, as such contributing to the diversity of MGEs in the population




Lopez-Sanchez MJ., Sauvage E., Da Cunha V., Clermont D., Ratsima-Hariniaina E., Gonzalez-Zorn B., Poyart C., Rosinski-Chupin I. y Glaser P.




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The highly dynamic CRISPR1 system of Streptococcus agalactiae controls the diversity of its mobilome

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The highly dynamic CRISPR1 system of Streptococcus agalactiae controls the diversity of its mobilome



Participantes:

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Institut PasteurUnité de Biologie des Bactéries Pathogènes à Gram Positif. Département de Microbiologie. Institut Pasteur.

Institut PasteurCollection de l´Institut Pasteur. Institut Pasteur.

Institut Pasteur de MadagascarInstitut Pasteur de Madagascar (IPM).

Université Sorbonne Paris DescartesInstitut Cochin. Université Sorbonne Paris Descartes.

Centre National de Référence des Streptocoques (CNR-Strep). Groupe Hospitalier Paris Centre Cochin-Hôtel Dieu-Broca (AP-HP).







Molecular Microbiology
FACTOR YEAR Q
4.961 2012

NLMID: 8712028

PMID: 22834929

ISSN: 0950-382X



TÍTULO: The highly dynamic CRISPR1 system of Streptococcus agalactiae controls the diversity of its mobilome


REVISTA: Mol. Microbiol.


NUMERACIÓN: 85(6):1057-1071


AÑO: 2012


EDITORIAL: Blackwell Scientific Publications


AUTORES: Lopez-Sanchez MJ., Sauvage E., Da Cunha V., Clermont D., Ratsima-Hariniaina E., Gonzalez-Zorn B., Poyart C., Rosinski-Chupin I. and Glaser P.


PARTICIPANTES VISAVET


Bruno González Zorn

DOI: https://doi.org/10.1111/j.1365-2958.2012.08172.x...


CITA ESTA PUBLICACIÓN:

Lopez-Sanchez MJ., Sauvage E., Da Cunha V., Clermont D., Ratsima-Hariniaina E., Gonzalez-Zorn B., Poyart C., Rosinski-Chupin I. y Glaser P. The highly dynamic CRISPR1 system of Streptococcus agalactiae controls the diversity of its mobilome. Molecular Microbiology. 85(6):1057-1071. 2012. (A). ISSN: 0950-382X. DOI: 10.1111/j.1365-2958.2012.08172.x


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