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Validation of a real-time polymerase-chain reaction (PCR) to detect Leishmania infantum in Leporidae

Poster presentado en 4th Med-Vet-Net Annual Scientific Meeting 2015 (Global Challenges in Zoonoses: Combating Emerging Threats using a One-Health Approach)

8 de octubre de 2015

Ortega-García MV., Moreno I., Dominguez M. y Garcia N.

In the Mediterranean basin, where Leishmaniasis is an endemic disease, Leishmania infantum is the main species implicated and the dog its main domestic reservoir. However, in recent years Leporidae have been described as competent reservoirs. A rapid and accurate identification method of L. infantum is essential to improve surveillance programs in wild animals and real-time PCR offers these advantages over conventional PCR.
The aim of the study is to validate a real-time PCR assay described for dogs in blood, to detect L. infantum in different samples of 226 wild rabbits and 70 hares (Leporidae) from Madrid area and compare it to nested PCR.
A total of 71 rabbits (32.13%, 95% CI: 25.97-38.28) and 8 hares (11.43%, 95% CI: 3.98-18.88), and 178 rabbits (87.68%, 95% CI: 83.16-92.21) and 47 hares (67.14%, 95% CI: 56.14-78.15) were positive in spleen and skin samples, respectively by real-time PCR; whereas, 5 rabbits (2%, 95% CI: 0-4) and no hares, and 26 rabbits (11.50%, 95% CI: 7.41-15.8) and 1 hare (1.45%, 95% CI: 0-4.27) were positive in spleen and skin samples, respectively by nested PCR. Furthermore, 21 rabbits (70%, 95% CI: 53.60-86.4) and 17 hares (56.67%, 95% CI: 38.93-74.4) were positive in hair samples by real-time PCR.
The assay is more sensitive than nested PCR, as it has been probed in dogs, with a limit of detection of 31, 35 pg/reaction of L. infantum genomic DNA (0.0854 parasites), but lower than it had in dogs (0.001 parasites), so it could be considered adequate for diagnosis of L.infantum infection in wild Leporidae





Participantes:

Ministerio de DefensaInstituto Tecnológico La Marañosa (ITM). Ministerio de Defensa.

Ministerio de DefensaInstituto Nacional de Técnica Aeroespacial (INTA). Ministerio de Defensa.

Instituto de Salud Carlos IIIServicio de Inmunología. Centro Nacional de Microbiología (CNM). Instituto de Salud Carlos III (ISCIII).

Universidad ComplutenseServicio de Zoonosis Emergentes, de Baja Prevalencia y Agresivos Biológicos (NED). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).


Enlace a 4th Med-Vet-Net Annual Scientific Meeting 2015 (Global Challenges in Zoonoses: Combating Emerging Threats using a One-Health Approach)





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4th Med-Vet-Net Annual Scientific Meeting 2015 (Global Challenges in Zoonoses: Combating Emerging Threats using a One-Health Approach)


4th Med-Vet-Net Annual Scientific Meeting 2015 (Global Challenges in Zoonoses: Combating Emerging Threats using a One-Health Approach)
7-9 octubre de 2015
París
Francia

TÍTULO: Validation of a real-time polymerase-chain reaction (PCR) to detect Leishmania infantum in Leporidae


TIPO: Comunicación en póster


AUTORES: Ortega-García MV., Moreno I., Dominguez M. y Garcia N.


PARTICIPANTES VISAVET


First
Mª Victoria Ortega García
Last
Nerea García Benzaquén

FECHA: 8 de octubre de 2015



CITA ESTA COMUNICACIÓN:

Ortega-García MV., Moreno I., Dominguez M. y Garcia N. Validation of a real-time polymerase-chain reaction (PCR) to detect Leishmania infantum in Leporidae. 4th Med-Vet-Net Annual Scientific Meeting 2015 (Global Challenges in Zoonoses: Combating Emerging Threats using a One-Health Approach), Med-Vet-Net Association, París, Francia, 8 de octubre de 2015. (Comunicación en póster)


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