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Assessment of in vivo and in vitro tuberculosis diagnostic tests in Mycobacterium caprae naturally infected caprine flocks

Investigation published in Preventive Veterinary Medicine

July 1st, 2011

Caprine tuberculosis in Spain is mainly caused by Mycobacterium caprae although the progression of the disease and lesion severity is similar to that caused by Mycobacterium bovis. In this study, the sensitivity of the gamma-interferon (IFN-γ) assay using an antigen cocktail containing early secretory antigenic target-6kDa (ESAT-6) and culture filtrate protein 10 (CFP-10) peptides for stimulation was determined and compared with those obtained in single intradermal tuberculin (SIT) and single intradermal cervical comparative tuberculin (SICCT) tests and IFN-γ assay using purified protein derivative (PPD) in three different flocks infected with M. caprae under different epidemiological conditions. Correlation between specific IFN-γ production and severity of lesions was also evaluated. Sensitivities of the diagnostic tests varied greatly in the three flocks studied, with higher values in those where higher lesion scores were observed. The results show that IFN-γ assay applied in goats using PPD or the ESAT-6/CFP-10 peptides cocktail for stimulation yielded similar sensitivity values. A significant yet weak positive correlation between specific IFN-γ production and lesion scores was detected after the stimulation with PPDs (p=0.004) whereas when the blood samples were stimulated with ESAT-6/CFP-10 peptides, the correlation was not significant (p>0.05). Therefore, specific-IFN-γ production after the stimulation with PPDs or ESAT-6/CFP-10 was not an accurate indicator of lesion severity in naturally tuberculosis infected goats with M. caprae




Bezos J., Alvarez J., de Juan L., Romero B., Rodriguez-Campos S., Fernandez de Mera IG., Hewinson RG., Vordermeier M., Mateos A., Dominguez L. and Aranaz A.




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Assessment of in vivo and in vitro tuberculosis diagnostic tests in Mycobacterium caprae naturally infected caprine flocks

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Assessment of in vivo and in vitro tuberculosis diagnostic tests in Mycobacterium caprae naturally infected caprine flocks


Participants:

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Gobierno de Castilla-La ManchaInstituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).

Veterinary Laboratories AgencyVeterinary Laboratories Agency.







Preventive Veterinary Medicine
FACTOR YEAR Q
2.046 2011

NLMID: 8217463

PMID: 21546104

ISSN: 0167-5877



TITLE: Assessment of in vivo and in vitro tuberculosis diagnostic tests in Mycobacterium caprae naturally infected caprine flocks


JOURNAL: Prev. Vet. Med.


NUMERACIÓN: 100(3-4):187-92


AÑO: 2011


PUBLISHER: Elsevier Science Publishers


AUTHORS: Bezos J., Alvarez J., de Juan L., Romero B., Rodriguez-Campos S., Fernandez de Mera IG., Hewinson RG., Vordermeier M., Mateos A., Dominguez L. and Aranaz A.


VISAVET PARTICIPANTS


First
Javier Bezos Garrido
2nd
Julio Álvarez Sánchez
3rd
Lucía de Juan Ferré
4th
Beatriz Romero Martínez
10
Lucas Domínguez Rodríguez

DOI: https://doi.org/ 10.1016/j.prevetmed.2011.03.012


CITE THIS PUBLICATION:

Bezos J., Alvarez J., de Juan L., Romero B., Rodriguez-Campos S., Fernandez de Mera IG., Hewinson RG., Vordermeier M., Mateos A., Dominguez L. and Aranaz A. Assessment of in vivo and in vitro tuberculosis diagnostic tests in Mycobacterium caprae naturally infected caprine flocks. Preventive Veterinary Medicine. 100(3-4):187-92. 2011. (A). ISSN: 0167-5877. DOI: 10.1016/j.prevetmed.2011.03.012


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