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Colistin selection of the mcr-1 gene in broiler chicken intestinal microbiota

Investigation article published in Antibiotics

June 5th, 2021

: Colistin has a long story of safe use in animals for the treatment and prevention of certain bacterial diseases. Nevertheless, the first description of the mcr-1 gene showed that colistin resistance can spread by horizontal gene transfer and changed the landscape. This study aimed to assess the effect of colistin administration on the dispersion of resistance in the microbiota of day-old broiler chicks and how the presence of mcr-1 genes influences the spread of colistin resistance determinants. In this study, 100 one-day-old chicks were divided into four groups of 25 animals (G1, G2, G3, and G4). Animals from G3/G4 were challenged with mcr-1-carrying Salmonella (day 7), while colistin (600 mg/L) was administered daily to G2/G4 animals through drinking water (from day 8 to day 15). Two quantitative PCR assays were performed to compare the amount of Salmonella and mcr-1 that were present in the caecal samples. We observed that levels of mcr-1 were higher in G3/G4 animals, especially G4, due to the spread of mcr-1-carrying Salmonella. On day 21, Salmonella levels decreased in G4, reaching similar values as those for G3, but mcr-1 levels remained significantly higher, suggesting that colistin may accelerate the spreading process when mcr-1-carrying bacteria reach the gut




Miguela-Villoldo P., Moreno MA., Rebollada A., Rodriguez-Bertos A., Hernandez M., Rodriguez-Lazaro D., Gallardo A., Quesada A., Goyache J., Dominguez L. and Ugarte-Ruiz M..




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Colistin selection of the mcr-1 gene in broiler chicken intestinal microbiota

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Colistin selection of the mcr-1 gene in broiler chicken intestinal microbiota



Participants:

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Medicina y Cirugía Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Instituto Tecnológico Agrario de Castilla y LeónLaboratorio de Biología Molecular y Microbiología. Instituto Tecnológico Agrario de Castilla y León (ITACyL).

Universidad de BurgosFacultad de Ciencias. Universidad de Burgos (UBU).

Universidad de ExtremaduraDepartamento de Bioquímica, Biología Molecular y Genética. Facultad de Veterinaria. Universidad de Extremadura (UNEX).

Universidad de ExtremaduraInstituto Universitario de Biotecnología Ganadera y Cinegética (INBIO G+C). Universidad de Extremadura (UNEX).







Antibiotics
FACTOR YEAR Q
4.639 2020

PMID: 34198813

ISSN: 2079-6382



TITLE: Colistin selection of the mcr-1 gene in broiler chicken intestinal microbiota


JOURNAL: Antibiotics-Basel


NUMERACIÓN: 10:677


AÑO: 2021


PUBLISHER: MDPI


AUTHORS: Miguela-Villoldo P., Moreno MA., Rebollada A., Rodriguez-Bertos A., Hernandez M., Rodriguez-Lazaro D., Gallardo A., Quesada A., Goyache J., Dominguez L. and Ugarte-Ruiz M..


VISAVET PARTICIPANTS


First
Pedro Miguela Villoldo
2nd
Miguel Ángel Moreno Romo
3rd
Agustín Miguel Rebollada Merino
4th
Antonio Manuel Rodríguez Bertos
9th
Joaquín Goyache Goñi
10
Lucas Domínguez Rodríguez
Last
María Ugarte Ruiz

DOI: https://doi.org/ 10.3390/antibiotics10060677


CITE THIS PUBLICATION:

Miguela-Villoldo P., Moreno MA., Rebollada A., Rodriguez-Bertos A., Hernandez M., Rodriguez-Lazaro D., Gallardo A., Quesada A., Goyache J., Dominguez L. and Ugarte-Ruiz M. Colistin selection of the mcr-1 gene in broiler chicken intestinal microbiota. Antibiotics. 10:677. 2021. (A). ISSN: 2079-6382. DOI: 10.3390/antibiotics10060677


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