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Detection and isolation of Campylobacter from different matrices using five protocols

Poster presented in AHVLA International Conference 2011

September 15th, 2011

Ugarte-Ruiz M., Gomez S., Garcia M., Comeron MC., Castilla C., Porrero MC. and Dominguez L.

In 2009, campylobacteriosis was the most commonly reported foodborne zoonoses with 198.252 human cases notified and around nine million non-reported cases each year. The most frequently reported Campylobacter species was Campylobacter jejuni (36.4 %), while the confirmed cases of Campylobacter coli accounted for 2.5% (EFSA 2011).
We have evaluated different isolation and detection protocols, including ISO 10272: 2006- part 1 recommended for detection of Campylobacter in foods. We tested four different matrices: faeces, neck skin, packed fresh meat and sewage water (n=114). The isolation and identification of Campylobacter using conventional methods is slow and laborious, owing to its fragility and special culture requirement, including a microaerobic atmosphere. Moreover, the detection of Campylobacter is often difficult as the culture conditions need to be selective enough to avoid overgrowth of competitive organisms. Four different microbiological methods were compared, including three enrichment media; Bolton, Preston and Campyfood broths. In addition, two selective plating agars were evaluated; modified charcoal cefoperazone deoxycholate (mCCDA) and Campyfood agar (CFA). These methods were compared to a real time PCR assay, which yielded the highest number of positive samples (112/114; 98.24%). Regarding to microbiological techniques, a lesser detection ability was observed (98/114; 85.96%). Herein, we describe the best protocols for the isolation of Campylobacter from different matrices and an effective method for all of them. We have attained the isolation of a high number of Campylobacter strains which represents an essential source for our ulterior molecular characterization studies




Participants:

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).


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Detection and isolation of Campylobacter from different matrices using five protocols


Link to AHVLA International Conference 2011





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AHVLA International Conference 2011


AHVLA International Conference 2011
September 13rd-15th, 2011
London
United Kingdom

TITLE: Detection and isolation of Campylobacter from different matrices using five protocols


TYPE: Poster communication


AUTHORS: Ugarte-Ruiz M., Gomez S., Garcia M., Comeron MC., Castilla C., Porrero MC. and Dominguez L.


VISAVET PARTICIPANTS


First
María Ugarte Ruiz
3rd
María García Martín
Last
Lucas Domínguez Rodríguez

DATE: September 15th, 2011


CITE THIS COMMUNICATION:

Ugarte-Ruiz M., Gomez S., Garcia M., Comeron MC., Castilla C., Porrero MC. and Dominguez L. Detection and isolation of Campylobacter from different matrices using five protocols. AHVLA International Conference 2011, Animal Health and Veterinary Laboratories Agency, London, United Kingdom, September 15th, 2011. (Poster communication)


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