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Evaluation of a new enzyme-linked immunosorbent assay for the diagnosis of tuberculosis in goat milk

Investigation published in Research in Veterinary Science

February 1st, 2020

Caprine tuberculosis (TB) is a zoonosis with sanitary and economic repercussions. Caprine TB control programs are based on a test and cull strategy using the intradermal tuberculin tests and slaughterhouse surveillance. However, this approach is not always feasible and may have a limited sensitivity under specific circumstances. In this study, performance of a new experimental test based on the P22 protein complex (P22 ELISA) was evaluated in two TB-infected herds using milk and serum samples and compared with cell-based diagnostic tests. Samples from a low (n = 62, herd 1) and a high (n = 52, herd 2) TB prevalence herd were selected. Moreover, bulk tank
milk samples from both herds were analysed using the P22 ELISA. At the end of the study, a group of animals (n = 21) was euthanized and subjected to post-mortem analysis and bacteriological culture. Significant differences (p < .001) on the qualitative and quantitative (ODs) results were observed between herds using both serum and milk samples in the P22 ELISA. The correlation observed in the quantitative results obtained in serum and milk samples was very strong in animals from flock 2 (rs = 0.91) and moderate in animals from flock 1 (rs = 0.46). Among the slaughtered animals, the P22 ELISA detected a higher proportion of lesion-culture positive animals
than cell-based diagnostic tests (61.9 and 66.7% using milk and serum samples, respectively). The P22 ELISA using milk samples demonstrated a similar sensitivity compared with serum samples, suggesting it might be a valuable test for TB control in dairy goats




Roy A., Infantes-Lorenzo JA., Dominguez M., Moreno I., Perez-Sancho M., Garcia N., Garcia-Seco T., Alvarez J., Romero B., Gortazar C., de Juan L., Dominguez L. and Bezos J..




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Evaluation of a new enzyme-linked immunosorbent assay for the diagnosis of tuberculosis in goat milk

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Evaluation of a new enzyme-linked immunosorbent assay for the diagnosis of tuberculosis in goat milk


Participants:

BIOFABRI, S.L.BIOFABRI, S.L..

Instituto de Salud Carlos IIIServicio de Inmunología. Centro Nacional de Microbiología (CNM). Instituto de Salud Carlos III (ISCIII).

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Gobierno de Castilla-La ManchaSanidad y Biotecnología (SaBio). Instituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).

Universidad ComplutenseServicio de Ensayos Clínicos (SEC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).







Research in Veterinary Science
FACTOR YEAR Q
1.751 2018

NLMID: 401300

PMID: 31835123

ISSN: 0034-5288



TITLE: Evaluation of a new enzyme-linked immunosorbent assay for the diagnosis of tuberculosis in goat milk


JOURNAL: Res. Vet. Sci.


NUMERACIÓN: 128:217-223


AÑO: 2020


PUBLISHER: Elsevier


AUTHORS: Roy A., Infantes-Lorenzo JA., Dominguez M., Moreno I., Perez-Sancho M., Garcia N., Garcia-Seco T., Alvarez J., Romero B., Gortazar C., de Juan L., Dominguez L. and Bezos J..


VISAVET PARTICIPANTS


5th
Marta Pérez Sancho
6th
Nerea García Benzaquén
7th
Teresa García-Seco Romero
8th
Julio Álvarez Sánchez
9th
Beatriz Romero Martínez
11th
Lucía de Juan Ferré
12th
Lucas Domínguez Rodríguez
Last
Javier Bezos Garrido

DOI: https://doi.org/ 10.1016/j.rvsc.2019.12.009


CITE THIS PUBLICATION:

Roy A., Infantes-Lorenzo JA., Dominguez M., Moreno I., Perez-Sancho M., Garcia N., Garcia-Seco T., Alvarez J., Romero B., Gortazar C., de Juan L., Dominguez L. and Bezos J. Evaluation of a new enzyme-linked immunosorbent assay for the diagnosis of tuberculosis in goat milk. Research in Veterinary Science. 128:217-223. 2020. (A). ISSN: 0034-5288. DOI: 10.1016/j.rvsc.2019.12.009


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