Veterinary Research publish this investigation article

October 15^th, 2019

Effective vaccines against tuberculosis (TB) are needed in order to prevent TB transmission in human and animal populations. Evaluation of TB vaccines may be facilitated by using reliable animal models that mimic host pathophysiology and natural transmission of the disease as closely as possible. In this study, we evaluated the immunogenicity and efficacy of two attenuated vaccines, BCG and MTBVAC, after each was given to 17 goats (2 months old) and then exposed for 9 months to goats infected with M. caprae. In general, MTBVAC-vaccinated goats showed higher interferon-gamma release than BCG vaccinated goats in response to bovine protein purified derivative and ESAT-6/CFP-10 antigens and the response was significantly higher than that observed in the control group until challenge. All animals showed lesions consistent with TB at the end of the study. Goats that received either vaccine showed significantly lower scores for pulmonary lymph nodes and total lesions than unvaccinated controls. Both MTBVAC and BCG vaccines proved to be immunogenic and effective in reducing severity of TB pathology caused by M. caprae. Our model system of natural TB transmission may be useful for evaluating and optimizing vaccines

Roy A., Tome-Sanchez I., Romero B., Lorente-Leal V., Infantes-Lorenzo JA., Dominguez M., Martin C., Aguilo N., Puentes E., Rodriguez E., de Juan L., Risalde MA., Gortazar C., Dominguez L. and Bezos J..

Evaluation of the immunogenicity and efficacy of BCG and MTBVAC vaccines using a natural transmission model of tuberculosis

Evaluation of the immunogenicity and efficacy of BCG and MTBVAC vaccines using a natural transmission model of tuberculosis

	BIOFABRI, S.L..
	Servicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).
	Centro Nacional de Microbiología (CNM). Instituto de Salud Carlos III (ISCIII).
	Departamento de Microbiología, Medicina Preventiva y Salud Pública. Facultad de Medicina. Universidad de Zaragoza (UNIZAR).
	Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES). Instituto de Salud Carlos III (ISCIII).
	Servicio de Microbiología. Hospital Universitario Miguel Servet.
	Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).
	Departamento de Anatomía y Anatomía Patológica Comparadas. Facultad de Veterinaria. Universidad de Córdoba (UCO).
	Unidad de Enfermedades Infecciosas. Hospital Universitario Reina Sofía de Córdoba. Instituto Maimonides de Investigación Biomédica de Cordoba.
	Sanidad y Biotecnología (SaBio). Instituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).
	Servicio de Ensayos Clínicos (SEC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).