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Factors affecting the performance of P22 ELISA for the diagnosis of caprine tuberculosis in milk samples

Investigation article published in Research in Veterinary Science

July 1st, 2022

Caprine tuberculosis (TB) is a zoonosis caused by members of the Mycobacterium tuberculosis complex (MTBC). Caprine TB eradication programmes are based mainly on intradermal tuberculin tests and slaughterhouse surveillance. However, the use of serological test has been extended as a potential diagnostic tool in goats through the use of serum, plasma, or even milk samples. Milk production and the antibodies (Ab) present in milk can vary depending on several circumstances. In the present study, different factors that may affect the performance of humoral TB diagnosis were analysed using goat milk samples: 1) lactation stage, 2) a recent previous skin test (booster effect) and 3) the effect of freeze-thaw cycles on milk samples preserved with azidiol. TB-infected animals (n = 44) were selected to evaluate the evolution of the Ab levels during the 6-month lactation period, along with its potential effect on the P22 ELISA results. In general, no significant changes (p = 0.079) were observed throughout the study as regards Ab levels in milk samples between consecutive analysis although the reactivity to P22 ELISA decreased when samplings were performed at the last two months of the lactation. Regarding the booster effect, the quantitative results showed a significant variation (p < 0.001) for both milk and serum samples when serological tests were carried out 15 days after the skin test. Finally, there were no significant differences (p = 0.99) in the P22 ELISA results when using milk samples preserved with azidiol that had undergone freeze-thaw cycles




Ortega J., Infantes-Lorenzo JA., Roy A., de Juan L., Romero B., Moreno I., Dominguez M., Dominguez L. and Bezos J..




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Factors affecting the performance of P22 ELISA for the diagnosis of caprine tuberculosis in milk samples

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Factors affecting the performance of P22 ELISA for the diagnosis of caprine tuberculosis in milk samples



Participants:

Universidad ComplutenseServicio de Micobacterias (MYC). Servicio de Ensayos Clínicos (SEC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Instituto de Salud Carlos IIIServicio de Inmunología. Centro Nacional de Microbiología (CNM). Instituto de Salud Carlos III (ISCIII).







Research in Veterinary Science
FACTOR YEAR Q
2.554 2021

NLMID: 401300

PMID: 35151157

ISSN: 0034-5288



TITLE: Factors affecting the performance of P22 ELISA for the diagnosis of caprine tuberculosis in milk samples


JOURNAL: Res Vet Sci


NUMERACIÓN: 145:40-45


AÑO: 2022


PUBLISHER: Elsevier


AUTHORS: Ortega J., Infantes-Lorenzo JA., Roy A., de Juan L., Romero B., Moreno I., Dominguez M., Dominguez L. and Bezos J..


VISAVET PARTICIPANTS


First
Javier Ortega Martín
4th
Lucía de Juan Ferré
5th
Beatriz Romero Martínez
8th
Lucas Domínguez Rodríguez
Last
Javier Bezos Garrido

DOI: https://doi.org/10.1016/j.rvsc.2022.02.008


CITE THIS PUBLICATION:

Ortega J., Infantes-Lorenzo JA., Roy A., de Juan L., Romero B., Moreno I., Dominguez M., Dominguez L. and Bezos J. Factors affecting the performance of P22 ELISA for the diagnosis of caprine tuberculosis in milk samples. Research in Veterinary Science. 145:40-45. 2022. (A). ISSN: 0034-5288. DOI: 10.1016/j.rvsc.2022.02.008


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