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Factors influencing the performance of an interferon-y assay for diagnosis of tuberculosis in goats

Investigation article published in The Veterinary Journal

October 1st, 2011

The interferon-gamma (IFN-gamma) assay is an effective tool for the diagnosis of tuberculosis in goats. The objectives of this study were to evaluate factors that might affect assay performance: (1) 0.1%, 0.3% and 0.5% phenol in purified protein derivative (PPD, tuberculin); (2) dialysis of PPD; and (3) delaying antigenic stimulation of blood samples for 8, 16 and 24 h after collection. The assay was performed in duplicate with two cut-off points. Dialysis of PPD reduced test sensitivity, whereas the concentration of phenol did not significantly affect test outcome. Delaying antigenic stimulation of samples >8 h resulted in a reduction in test sensitivity, compromising the capacity of the assay to detect infected animals. Performing the assay in duplicate was unnecessary, which has implications for reducing assay costs. These findings will facilitate the effective application of the IFN-gamma assay as an ancillary test in tuberculosis eradication programmes in goats.




Bezos J., Alvarez J., de Juan L., Romero B., Rodriguez-Campos S., Castellanos E., Saez JL., Mateos A., Dominguez L. and Aranaz A.




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Factors influencing the performance of an interferon-y assay for diagnosis of tuberculosis in goats

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Factors influencing the performance of an interferon-y assay for diagnosis of tuberculosis in goats


Participants:

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Gobierno de Castilla-La ManchaInstituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).

Dirección General de Recursos Agrícolas y Ganaderos.

Ministerio de Medio Ambiente y Medio Rural y MarinoMinisterio de Medio Ambiente y Medio Rural y Marino (MARM).







The Veterinary Journal
FACTOR YEAR Q
2.239 2011

NLMID: 9706281

PMID: 21050783

ISSN: 1090-0233



TITLE: Factors influencing the performance of an interferon-y assay for diagnosis of tuberculosis in goats


JOURNAL: Vet. J.


NUMERACIÓN: 190(1):131-135


AÑO: 2011


PUBLISHER: Elsevier SCI LTD


AUTHORS: Bezos J., Alvarez J., de Juan L., Romero B., Rodriguez-Campos S., Castellanos E., Saez JL., Mateos A., Dominguez L. and Aranaz A.


VISAVET PARTICIPANTS


First
Javier Bezos Garrido
2nd
Julio Álvarez Sánchez
3rd
Lucía de Juan Ferré
4th
Beatriz Romero Martínez
9th
Lucas Domínguez Rodríguez

DOI: https://doi.org/ 10.1016/j.tvjl.2010.09.026


CITE THIS PUBLICATION:

Bezos J., Alvarez J., de Juan L., Romero B., Rodriguez-Campos S., Castellanos E., Saez JL., Mateos A., Dominguez L. and Aranaz A. Factors influencing the performance of an interferon-y assay for diagnosis of tuberculosis in goats. The Veterinary Journal. 190(1):131-135. 2011. (A). ISSN: 1090-0233. DOI: 10.1016/j.tvjl.2010.09.026


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