Home \ Research \ Scientific publications \


Histopathologic and immunohistochemical findings in the placentas and fetuses of domestic swine naturally infected with Brucella suis biovar 2

Journal of Veterinary Diagnostic Investigation publish this investigation article

May 23rd, 2023

Porcine brucellosis, which is caused by Brucella suis biovar (bv) 2, is a re-emerging disease that causes reproductive problems in pigs in Europe. The pathogenesis and lesions of B. suis intrauterine infection are poorly characterized; characterization could facilitate the diagnosis and investigation of porcine brucellosis. We collected samples of placentas and fetuses for histologic and microbiologic studies during an outbreak of abortions on a pig-breeding farm in Spain. Brucella was cultured from the vaginal swabs obtained from sows that had aborted, some placentas, and fetal tissues (spleen, liver, lung, gastric content); molecular testing confirmed B. suis bv 2 infection. Histologically, there was necrotizing and hemorrhagic placentitis; suppurative hepatitis; lymphoid depletion and sinusoidal histiocytosis in the spleen, lymph nodes, and thymus; and bronchointerstitial pneumonia. Hemorrhages were observed in the umbilical cord, heart, kidneys, and brain. We detected Brucella by immunohistochemistry (IHC) in all of the placentas and fetal organs studied, specifically in the trophoblasts of the chorionic epithelium, in the cytoplasm of macrophages in the chorionic stroma, and extracellularly in necrotic debris. Furthermore, we assessed the lymphocyte population in the placentas through the use of IHC (anti-CD3, anti-Pax5 antibodies),
revealing that the lymphocytic response was composed of T cells but not B cells




Rebollada A., Garcia-Seco T., Perez-Sancho M., Dominguez L. and Rodriguez-Bertos A..




See this article
Histopathologic and immunohistochemical findings in the placentas and fetuses of domestic swine naturally infected with Brucella suis biovar 2

See it on NLM PubMed
Histopathologic and immunohistochemical findings in the placentas and fetuses of domestic swine naturally infected with Brucella suis biovar 2



Participants:

Universidad ComplutenseServicio de Patología y Veterinaria Forense (SAP). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Medicina y Cirugía Animal. Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







Journal of Veterinary Diagnostic Investigation
FACTOR YEAR Q
1.200 2023

NLMID: 9011490

PMID: 36988301

ISSN: 1040-6387



TITLE: Histopathologic and immunohistochemical findings in the placentas and fetuses of domestic swine naturally infected with Brucella suis biovar 2


JOURNAL: J Vet Diagn Invest


NUMERACIÓN: 35(3):258-265


AÑO: 2023


PUBLISHER: American Association Of Veterinary Laboratory Diagnosticians


AUTHORS: Rebollada A., Garcia-Seco T., Perez-Sancho M., Dominguez L. and Rodriguez-Bertos A..


2nd
María Teresa García-Seco Romero
3rd
Marta Pérez Sancho
4th
Lucas Domínguez Rodríguez
Last
Antonio Manuel Rodríguez Bertos

DOI: https://doi.org/10.1177/10406387231163867


CITE THIS PUBLICATION:

Rebollada A., Garcia-Seco T., Perez-Sancho M., Dominguez L. and Rodriguez-Bertos A. Histopathologic and immunohistochemical findings in the placentas and fetuses of domestic swine naturally infected with Brucella suis biovar 2. Journal of Veterinary Diagnostic Investigation . 35(3):258-265. 2023. (A). ISSN: 1040-6387. DOI: 10.1177/10406387231163867


UNITS: