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Interference of paratuberculosis with the diagnosis of tuberculosis in a goat flock with a natural mixed infection

Investigation article published in Veterinary Microbiology

April 1st, 2008

Detection of infected animals is a key step in eradication programs of tuberculosis. Paratuberculosis infection has been demonstrated to compromise the specificity of the diagnostic tests. However, its effect on their sensitivity has not been clarified. In the present study, skin tests and the interferon-gamma (IFN-gamma) assay were evaluated in a goat flock (n=177) with a mixed tuberculosis-paratuberculosis infection in order to assess the possible effect of paratuberculosis on their sensitivity. Culture of mycobacteria was performed as the gold standard to determine the true infection status. All techniques showed lower sensitivities than previously described; the single intradermal tuberculin (SIT) test and the IFN-gamma assay detected 71% (62.4-78.6, 95% C.I.) of the infected animals; the single intradermal cervical comparative tuberculin (SICCT) test detected only 42.7% (34.1-51.7, 95% C.I.) of infected animals. The highest level of sensitivity was obtained when SIT test and IFN-gamma assay were combined in parallel (90.8%, 84.5-95.2, 95% C.I.). Sensitivities of the tests were also assessed by comparing animals suffering tuberculosis and animals with a mixed infection; tests were found to be more effective in the former group. Paratuberculosis seems to have a major effect in the sensitivity of the diagnostic tests under study, and therefore must be taken into account; in particular, the use of the SICCT test should be questioned when both tuberculosis and paratuberculosis are present




Alvarez J., de Juan L., Bezos J., Romero B., Saez JL., Reviriego FJ., Briones V., Moreno MA., Mateos A., Dominguez L. and Aranaz A.




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Interference of paratuberculosis with the diagnosis of tuberculosis in a goat flock with a natural mixed infection

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Interference of paratuberculosis with the diagnosis of tuberculosis in a goat flock with a natural mixed infection


Participants:

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

European CommissionDirectorate General for Health & Consumers. European Commission.

Ministerio de Agricultura, Pesca y AlimentaciónDirección General de Ganaderia. Ministerio de Agricultura, Pesca y Alimentación (MAPA).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







Veterinary Microbiology
FACTOR YEAR Q
2.370 2008

NLMID: 7705469

PMID: 17954015

ISSN: 0378-1135



TITLE: Interference of paratuberculosis with the diagnosis of tuberculosis in a goat flock with a natural mixed infection


JOURNAL: Vet. Microbiol.


NUMERACIÓN: 128(1-2):72-80


AÑO: 2008


PUBLISHER: Elsevier Science Publishers


AUTHORS: Alvarez J., de Juan L., Bezos J., Romero B., Saez JL., Reviriego FJ., Briones V., Moreno MA., Mateos A., Dominguez L. and Aranaz A.


VISAVET PARTICIPANTS


First
Julio Álvarez Sánchez
2nd
Lucía de Juan Ferré
3rd
Javier Bezos Garrido
4th
Beatriz Romero Martínez
7th
Víctor Briones Dieste
8th
Miguel Ángel Moreno Romo
10
Lucas Domínguez Rodríguez

DOI: https://doi.org/ 10.1016/j.vetmic.2007.08.034


CITE THIS PUBLICATION:

Alvarez J., de Juan L., Bezos J., Romero B., Saez JL., Reviriego FJ., Briones V., Moreno MA., Mateos A., Dominguez L. and Aranaz A. Interference of paratuberculosis with the diagnosis of tuberculosis in a goat flock with a natural mixed infection. Veterinary Microbiology. 128(1-2):72-80. 2008. (A). ISSN: 0378-1135. DOI: 10.1016/j.vetmic.2007.08.034


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