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MIRU-VNTR genotyping of Mycobacterium avium subspecies paratuberculosis revealed Type-specific differences at two component regulatory systems

Oral communication in 10th International Colloquium on Paratuberculosis

August 9th, 2009

Castellanos E., Romero B., Rodriguez-Campos S., Bezos J., de Juan L., Mateos A., Dominguez L. and Aranaz A.

In this report, we genotyped a collection of Spanish Mycobacterium avium subspecies paratuberculosis isolates with a combination of six mycobacterial interspersed repetitive units (MIRU) and variable number tandem repeats (VNTR) loci; MIRU-2, MIRU-3 (Bull et al., 2003), VNTR-25, VNTR-32, VNTR-292 (Thibault et al., 2007)
and VNTR-259 (this study). This research forms part of the EU project ParaTBtools.
For this purpose we screened 70 M. a. paratuberculosis Type II and III isolates, obtained from domestic livestock (goats and cattle) and two isolates recovered from wild animal species (fallow deer- Dama dama- and mouflon- Ovis orientalis musimon) throughout 22 different Spanish localities in a nine-year period.
The selected combination of MIRU-VNTR loci differentiated 17 different allelic variants within this population of M. a. paratuberculosis isolates, with a resulting discriminatory index (D) of 0.82. An interesting feature was the M. a. paratuberculosis Type-specific differences observed at the number of repeats at MIRU-3, located between genes SenX3 and RegX3, components of a regulatory system with a role in the virulence of Mycobacterium tuberculosis (Magdalena et al., 1998). In addition, M. a. paratuberculosis Type-specific number of tandem repeats was also obtained for the novel locus VNTR-259, which as in the case of MIRU-3, was located between a two component regulatory system, AfsQ1/AfsQ2, which has a role in the regulation of the antibiotic production strategies in Streptomyces (Shu et al., 2009)




Participants:

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).


Link to 10th International Colloquium on Paratuberculosis





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10th International Colloquium on Paratuberculosis


10th International Colloquium on Paratuberculosis
August 9th-14th, 2009
Minneapolis
United States

TITLE: MIRU-VNTR genotyping of Mycobacterium avium subspecies paratuberculosis revealed Type-specific differences at two component regulatory systems


TYPE: Oral communication


AUTHORS: Castellanos E., Romero B., Rodriguez-Campos S., Bezos J., de Juan L., Mateos A., Dominguez L. and Aranaz A.


VISAVET PARTICIPANTS


2nd
Beatriz Romero Martínez
4th
Javier Bezos Garrido
5th
Lucía de Juan Ferré
7th
Lucas Domínguez Rodríguez

DATE: August 9th, 2009


CITE THIS COMMUNICATION:

Castellanos E., Romero B., Rodriguez-Campos S., Bezos J., de Juan L., Mateos A., Dominguez L. and Aranaz A. MIRU-VNTR genotyping of Mycobacterium avium subspecies paratuberculosis revealed Type-specific differences at two component regulatory systems. 10th International Colloquium on Paratuberculosis, Department of Veterinary Population Medicine. College of Veterinary Medicine. University of Minnesota, Minneapolis, United States, August 9th, 2009. (Oral communication)


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