Comparative Immunology, Microbiology and Infectious Diseases publish this investigation article

November 1^st, 2022

The P22 ELISA was recently developed for the serodiagnosis of animal tuberculosis. Herein, the stability of the P22 antigen in different presentations and storage conditions, and the cross-reactivity with Corynebacterium pseudotuberculosis infection in small ruminants were evaluated. For the stability assay, serum samples from cows, sheep, goats, alpacas, badgers, and wild boar were used in the P22 ELISA. The cross-reactivity analysis used sera from sheep and goats with caseous lymphadenitis (CLA). Differences in the immune recognition of P22 were found when the antigen was stored at 40 °C, but without altering the negative or positive status of each sample. P22 ELISA presented 5.71 % cross-reactivity when CLA-positive sheep were evaluated, but no cross-reaction was observed among CLA-positive goat serum samples. This study showed that the P22 protein complex is stable under different formulations and temperatures, and that the assay presents a low cross-reactivity with CLA.
Keywords: Caseous lymphadenitis; Immunodiagnosis; Livestock; Mycobacterium tuberculosis complex; Wildlife; Zoonosis

Doria-Barral T., Infantes-Lorenzo JA., Moreno I., de Garnica-Garcia MG., Perez del Val B., Gortazar C., Meyer B., Portela RD., Dominguez L., Dominguez M. and Balseiro A.

P22 protein complex in the serodiagnosis of animal tuberculosis: Antigenic stability and cross-reactivity with Corynebacterium pseudotuberculosis infection

P22 protein complex in the serodiagnosis of animal tuberculosis: Antigenic stability and cross-reactivity with Corynebacterium pseudotuberculosis infection

	Laboratório de Imunologia e Biologia Molecular. Instituto de Ciências da Saúde. Universidade Federal do Recôncavo da Bahia.
	Servicio de Inmunología. Centro Nacional de Microbiología (CNM). Instituto de Salud Carlos III (ISCIII).
	Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad de León.
	Centre de Recerca en Sanitat Animal (CReSA). Universitat Autònoma de Barcelona (UAB). Instituto de Investigación y Tecnología Agroalimentarias (IRTA). Generalitat de Catalunya.
	Sanidad y Biotecnología (SaBio). Instituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).
	Servicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).
	Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).
	Departamento de Sanidad Animal. Instituto de Ganadería de Montaña. Consejo Superior de Investigaciones Científicas (CSIC).