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Persistence and molecular evolution of Mycobacterium bovis population from cattle and wildlife in Do?ana National Park revealed by genotype variation

Investigation published in Veterinary Microbiology

November 25th, 2008

The role of wildlife in tuberculosis epidemiology is being widely studied since it can affect the effectiveness of eradication campaigns in cattle. The health problem is enhanced when it concerns also wildlife welfare and biodiversity conservation. This study was performed to understand the epidemiology of Mycobacterium bovis population affecting livestock and wild animals in the Doñana National Park using bacteriology and molecular characterisation techniques. Tuberculosis research was performed on 1209 cattle and wild animals (artiodactyla and carnivore) collected over 6 years in the Park. One hundred and sixty-three animals were found to be infected with M. bovis, comprising 7.96% of the cattle and 20.53% of the wild animals tested. Spoligotyping revealed nine patterns, being SB1232 and SB1230 the most prevalent (77.30% and 15.34% of infected animals, respectively). MIRU-VNTR analysis of a selected panel of 92 isolates showed eight different profiles, including several spoligotypes within the same MIRU-VNTR profile. The discriminatory capacity of both techniques in this panel was similar. The results obtained by combination of both techniques corroborate that wildlife species are infected with the M. bovis strains which are more prevalent in cattle and reveal their persistence. Genotype variation between isolates strongly suggests micro-evolutionary events in the M. bovis population in the same area. This study in the Doñana National Park exposes the risk of introduction of domestic animals into wildlife areas when there is not a warranty of disease freedom, appropriate diagnostic techniques and control measures




Romero B., Aranaz A., Sandoval A., Alvarez J., de Juan L., Bezos J., Sanchez C., Galka M., Fernandez P., Mateos A. and Dominguez L..




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Persistence and molecular evolution of Mycobacterium bovis population from cattle and wildlife in Doñana National Park revealed by genotype variation

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Persistence and molecular evolution of Mycobacterium bovis population from cattle and wildlife in Doñana National Park revealed by genotype variation



Participants:

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Ministerio de Medio Ambiente y Medio Rural y MarinoEl acebuche. Ministerio de Medio Ambiente y Medio Rural y Marino (MARM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad de CórdobaDepartamento de Botánica, Ecología y Fisiología Vegetal. Facultad de Veterinaria. Universidad de Córdoba (UCO).







Veterinary Microbiology
FACTOR YEAR Q
2.370 2008

NLMID: 7705469

PMID: 18539410

ISSN: 0378-1135



TITLE: Persistence and molecular evolution of Mycobacterium bovis population from cattle and wildlife in Doñana National Park revealed by genotype variation


JOURNAL: Vet. Microbiol.


NUMERACIÓN: 132(1-2):87-95


AÑO: 2008


PUBLISHER: Elsevier Science Publishers


AUTHORS: Romero B., Aranaz A., Sandoval A., Alvarez J., de Juan L., Bezos J., Sanchez C., Galka M., Fernandez P., Mateos A. and Dominguez L..


VISAVET PARTICIPANTS


First
Beatriz Romero Martínez
4th
Julio Álvarez Sánchez
5th
Lucía de Juan Ferré
6th
Javier Bezos Garrido
Last
Lucas Domínguez Rodríguez

DOI: https://doi.org/10.1016/j.vetmic.2008.04.032


CITE THIS PUBLICATION:

Romero B., Aranaz A., Sandoval A., Alvarez J., de Juan L., Bezos J., Sanchez C., Galka M., Fernandez P., Mateos A. and Dominguez L. Persistence and molecular evolution of Mycobacterium bovis population from cattle and wildlife in Doñana National Park revealed by genotype variation. Veterinary Microbiology. 132(1-2):87-95. 2008. (A). ISSN: 0378-1135. DOI: 10.1016/j.vetmic.2008.04.032


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