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Proteomic characterisation of bovine and avian purified protein derivatives and identification of specific antigens for serodiagnosis of bovine tuberculosis

Investigation published in Clinical Proteomics

November 2nd, 2017

Bovine purified protein derivative (bPPD) and avian purified protein derivative (aPPD) are widely used for bovine tuberculosis diagnosis. However, little is known about their qualitative and quantitative characteristics, which makes their standardisation difficult. In addition, bPPD can give false-positive tuberculosis results because of sequence homology between Mycobacterium bovis (M. bovis) and M. avium proteins. Thus, the objective of this study was to carry out a proteomic characterisation of bPPD, aPPD and an immunopurified subcomplex from bPPD called P22 in order to identify proteins contributing to cross-reactivity among these three products in tuberculosis diagnosis




Infantes-Lorenzo JA., Moreno I., Risalde MA., Roy A., Villar M., Romero B., Ibarrola N., de la Fuente J., Puentes E., de Juan L., Gortazar C., Bezos J., Dominguez L. and Dominguez M.




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Proteomic characterisation of bovine and avian purified protein derivatives and identification of specific antigens for serodiagnosis of bovine tuberculosis

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Proteomic characterisation of bovine and avian purified protein derivatives and identification of specific antigens for serodiagnosis of bovine tuberculosis


Participants:

Instituto de Salud Carlos IIICentro Nacional de Microbiología (CNM). Instituto de Salud Carlos III (ISCIII).

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Gobierno de Castilla-La ManchaSanidad y Biotecnología (SaBio). Instituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).

MAEVA SERVET, S.L.MAEVA SERVET, S.L..

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad de SalamancaUnidad de Proteómica. Instituto de Biología Molecular y Celular del Cáncer. Universidad de Salamanca (USAL).

CZ Veterinaria, S.A.CZ Veterinaria, S.A. (CZV).

Oklahoma State UniversityDepartament of Veterinary Pathobiology. Center for Veterinary Health Sciences (CVHS). Oklahoma State University (OSU).







FACTOR YEAR Q
3.516 2017

PMID: 29142508

ISSN: 1542-6416



TITLE: Proteomic characterisation of bovine and avian purified protein derivatives and identification of specific antigens for serodiagnosis of bovine tuberculosis


JOURNAL: Clin Proteom


NUMERACIÓN: 14:36


AÑO: 2017


PUBLISHER: Springer International Publishing AG


AUTHORS: Infantes-Lorenzo JA., Moreno I., Risalde MA., Roy A., Villar M., Romero B., Ibarrola N., de la Fuente J., Puentes E., de Juan L., Gortazar C., Bezos J., Dominguez L. and Dominguez M.


VISAVET PARTICIPANTS


4th
Álvaro Roy Cordero
6th
Beatriz Romero Martínez
10
Lucía de Juan Ferré
12th
Javier Bezos Garrido
13th
Lucas Domínguez Rodríguez

DOI: https://doi.org/ 10.1186/s12014-017-9171-z


CITE THIS PUBLICATION:

Infantes-Lorenzo JA., Moreno I., Risalde MA., Roy A., Villar M., Romero B., Ibarrola N., de la Fuente J., Puentes E., de Juan L., Gortazar C., Bezos J., Dominguez L. and Dominguez M. Proteomic characterisation of bovine and avian purified protein derivatives and identification of specific antigens for serodiagnosis of bovine tuberculosis. Clinical Proteomics. 14:36. 2017. (A). ISSN: 1542-6416. DOI: 10.1186/s12014-017-9171-z


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