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Risk factors associated with negative in-vivo diagnostic results in bovine tuberculosis-infected cattle in Spain

BMC Veterinary Research publish this investigation article

January 13rd, 2014

Background
Despite great effort and investment incurred over decades to control bovine tuberculosis (bTB), it is still one of the most important zoonotic diseases in many areas of the world. Test-and-slaughter strategies, the basis of most bTB eradication programs carried out worldwide, have demonstrated its usefulness in the control of the disease. However, in certain countries, eradication has not been achieved due in part to limitations of currently available diagnostic tests. In this study, results of in-vivo and post-mortem diagnostic tests performed on

3,614 animals from 152 bTB-infected cattle herds (beef, dairy, and bullfighting) detected in 2007-2010 in the region of Castilla y Leon, Spain, were analyzed to identify factors associated with positive bacteriological results in cattle that were non-reactors to the single intradermal tuberculin test, to the interferon-gamma (IFN-gamma) assay, or to both tests applied in parallel (Test negative/Culture + animals, T-/C+). The association of individual factors (age, productive type, and number of herd-tests performed since the disclosure of the outbreak) with the bacteriology outcome (positive/negative) was analyzed using a mixed multivariate logistic regression model.

Results
The proportion of non-reactors with a positive post-mortem result ranged from 24.3% in the case of the SIT test to 12.9% (IFN-gamma with 0.05 threshold) and 11.9% (95% CI 9.9-11.4%) using both tests in parallel. Older (>4.5 years) and bullfighting cattle were associated with increased odds of confirmed bTB infection by bacteriology, whereas dairy cattle showed a significantly lower risk. Ancillary use of IFN-gamma assay reduced the proportion of T-/C + animals in high risk groups.

Conclusions
These results demonstrate the likelihood of positive bacteriological results in non-reactor cattle is influenced by individual epidemiological factors of tested animals. Increased surveillance on non-reactors with an increased probability of being false negative could be helpful to avoid bTB persistence, particularly in chronically infected herds. These findings may aid in the development of effective strategies for eradication of bTB in Spain.




Alvarez J., Perez AM., Marques S., Bezos J., Grau A., de la Cruz ML., Romero B., Saez JL., Esquivel MR., Martinez MC., Minguez O., de Juan L. and Dominguez L..




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Risk factors associated with negative in-vivo diagnostic results in bovine tuberculosis-infected cattle in Spain

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Risk factors associated with negative in-vivo diagnostic results in bovine tuberculosis-infected cattle in Spain



Participants:

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

University of California-DavisUniversity of California-Davis (UCDAVIS).

Facultad de Ciencias Veterinarias. Universidad Nacional de Rosario (UNR).

Junta de Castilla y LeónServicio de Sanidad Animal. Junta de Castilla y León.

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Comunidad de MadridInstituto Ramón y Cajal de Investigación Sanitaria (IRYCIS). Universidad Complutense (UCM). Universidad Autónoma de Madrid (UAM). Universidad de Alcalá (UAH). Salud Madrid. Comunidad de Madrid.







BMC Veterinary Research
FACTOR YEAR Q
1.777 2014

NLMID: 101249759

PMID: 24410926

ISSN: 1746-6148



TITLE: Risk factors associated with negative in-vivo diagnostic results in bovine tuberculosis-infected cattle in Spain


JOURNAL: BMC Vet. Res


NUMERACIÓN: 10(1):14


AÑO: 2014


PUBLISHER: BioMed Central


AUTHORS: Alvarez J., Perez AM., Marques S., Bezos J., Grau A., de la Cruz ML., Romero B., Saez JL., Esquivel MR., Martinez MC., Minguez O., de Juan L. and Dominguez L..


VISAVET PARTICIPANTS


First
Julio Álvarez Sánchez
4th
Javier Bezos Garrido
7th
Beatriz Romero Martínez
12th
Lucía de Juan Ferré
Last
Lucas Domínguez Rodríguez

DOI: https://doi.org/ 10.1186/1746-6148-10-14


CITE THIS PUBLICATION:

Alvarez J., Perez AM., Marques S., Bezos J., Grau A., de la Cruz ML., Romero B., Saez JL., Esquivel MR., Martinez MC., Minguez O., de Juan L. and Dominguez L. Risk factors associated with negative in-vivo diagnostic results in bovine tuberculosis-infected cattle in Spain. BMC Veterinary Research. 10(1):14. 2014. (A). ISSN: 1746-6148. DOI: 10.1186/1746-6148-10-14


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