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Specificity of serological test for detection of tuberculosis in cattle, goats, sheep and pigs under different epidemiological situations

BMC Veterinary Research publish this investigation article

March 1st, 2019

BACKGROUND:
Serum antibody detection has potential as a complementary diagnostic tool in animal tuberculosis (TB) control, particularly in multi-host systems. The objective of the present study was to assess the specificity (Sp) of an enzyme-linked immunosorbent assay (ELISA) based on the new multiprotein complex P22 for the detection of specific antibodies against the Mycobacterium tuberculosis complex (MTC) in the four most relevant domestic animals acting as MTC hosts: cattle, goat, sheep and pig. We used sera from an officially TB-free (OTF) country, Norway, and from a non-OTF one, Spain. The samples included sera from goats that had been vaccinated against M. avium subsp. paratuberculosis (MAP) and sheep from a herd in which Corynebacterium pseudotuberculosis had been isolated.
RESULTS:
In cattle, the Sp ranged from 92.5 (IC95% 90.7-94) to 99.4% (IC95% 98.3-99.8) depending on the cut-off used and the origin of the samples (Spain or Norway). Sp in cattle (cut-off point 100) was significantly higher (P < 0.05) for Norwegian samples. By contrast, Sp in goats was consistently low at the 100 cut-off [30.9 (CI95%23.4-39.5)-78% (CI95% 68.9-85)]. A higher cut-off of 150 improved Sp in Norwegian goats [97% (CI95% 91.6-99)], but still yielded a poor Sp of 56.1% (CI95% 47.3-64.6) in Spanish goats. In Norway at the 100 cut-off the Sp was 58.3 (CI95% 42.2-72.9) and 90.6% (CI95% 81-95.6) in MAP vaccinated and non-vaccinated goats, respectively, indicating interference due to MAP vaccination. Sp in sheep was between 94.4 (CI95% 91.7-96.3) and 100% (CI95% 96.3-100) depending on the cut-off and country, and no diagnostic interference due to infection with C. pseudotuberculosis was recorded. Sp in pigs was 100%, regardless the cut-off point applied, and no significant differences were observed between pigs from Norway and from Spain.

CONCLUSIONS:
Due to its excellent Sp in pigs and acceptable Sp in cattle and sheep, this ELISA may constitute a suitable option for TB screening at herd level, particularly in OTF-countries.
KEYWORDS:
Animal tuberculosis; Cattle; Goat; P22; Pig; Serum antibodies; Sheep




Infantes-Lorenzo JA., Moreno I., Roy A., Risalde MA., Balseiro A., de Juan L., Romero B., Bezos J., Puentes E., Akerstedt J., Tessema T., Gortazar C., Dominguez L. and Dominguez M.




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Specificity of serological test for detection of tuberculosis in cattle, goats, sheep and pigs under different epidemiological situations

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Specificity of serological test for detection of tuberculosis in cattle, goats, sheep and pigs under different epidemiological situations

Participants:

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Instituto de Salud Carlos IIIServicio de Inmunología. Centro Nacional de Microbiología (CNM). Instituto de Salud Carlos III (ISCIII).

CZ Veterinaria, S.A.CZ Veterinaria, S.A. (CZV).

Gobierno de Castilla-La ManchaSanidad y Biotecnología (SaBio). Instituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).

Universidad de CórdobaDepartamento de Anatomía y Anatomía Patológica Comparadas. Facultad de Veterinaria. Universidad de Córdoba (UCO).

Gobierno del Principado de AsturiasServicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA). Gobierno del Principado de Asturias.

Norwegian Veterinary InstituteNorwegian Veterinary Institute.







BMC Veterinary Research
FACTOR YEAR Q
1.958 2017

NLMID: 101249759

PMID: 30823881

ISSN: 1746-6148



TITLE: Specificity of serological test for detection of tuberculosis in cattle, goats, sheep and pigs under different epidemiological situations


JOURNAL: BMC Vet. Res


NUMERACIÓN: 15(1):70


AÑO: 2019


PUBLISHER: BioMed Central


AUTHORS: Infantes-Lorenzo JA., Moreno I., Roy A., Risalde MA., Balseiro A., de Juan L., Romero B., Bezos J., Puentes E., Akerstedt J., Tessema T., Gortazar C., Dominguez L. and Dominguez M.


VISAVET PARTICIPANTS


First
Jose Antonio Infantes Lorenzo
6th
Lucía de Juan Ferré
7th
Beatriz Romero Martínez
8th
Javier Bezos Garrido
13th
Lucas Domínguez Rodríguez

DOI: https://doi.org/ 10.1186/s12917-019-1814-z


CITE THIS PUBLICATION:

Infantes-Lorenzo JA., Moreno I., Roy A., Risalde MA., Balseiro A., de Juan L., Romero B., Bezos J., Puentes E., Akerstedt J., Tessema T., Gortazar C., Dominguez L. and Dominguez M. Specificity of serological test for detection of tuberculosis in cattle, goats, sheep and pigs under different epidemiological situations. BMC Veterinary Research. 15(1):70. 2019. (A). ISSN: 1746-6148. DOI: 10.1186/s12917-019-1814-z


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