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Splitting of a prevalent Mycobacterium bovis spoligotype by Variable Number Tandem Repeat Typing reveals high heterogeneity in an evolving clonal group

Journal of Clinical Microbiology publish this investigation article

November 1st, 2013

Mycobacterium bovis populations in countries with persistent bovine tuberculosis usually show a prevalent spoligotype with a wide geographical distribution. This study applied Mycobacterial Interspersed Repetitive Unit (MIRU) - Variable Number Tandem Repeat (VNTR) typing to a random panel of 115 M. bovis isolates representative of the most frequent spoligotype in the Iberian Peninsula, SB0121. VNTR typing targeted nine loci: ETR-A (alias VNTR2165), ETR-B (VNTR2461), ETR-D (MIRU4, VNTR580), ETR-E (MIRU31, VNTR3192), MIRU26 (VNTR2996), QUB11a (VNTR2163a), QUB11b (VNTR2163b), QUB26 (VNTR4052) and QUB3232 (VNTR3232). We found a high diversity among the studied isolates (discriminatory index D=0.9856) which were split into 65 different MIRU-VNTR types. An alternative MIRU-VNTR short format targeting only the four loci with the highest variability values was found to offer an equivalent discriminatory index. Minimum spanning trees using the MIRU-VNTR data showed the hypothetical evolution of an apparently clonal group. MIRU-VNTR analysis was also applied to isolates of 176 positive animals from 15 farms infected by M. bovis SB0121; in 10 farms it revealed the coexistence of two to five different MIRU types differing in one to six loci which highlights the frequent existence of undetected heterogeneity




Rodriguez-Campos S., Navarro Y., Romero B., de Juan L., Bezos J., Mateos A., Golby P., Smith NH., Hewinson RG., Dominguez L., Garcia de Viedma D. and Aranaz A.




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Splitting of a prevalent Mycobacterium bovis spoligotype by Variable Number Tandem Repeat Typing reveals high heterogeneity in an evolving clonal group

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Splitting of a prevalent Mycobacterium bovis spoligotype by Variable Number Tandem Repeat Typing reveals high heterogeneity in an evolving clonal group



Participants:

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

CEI Campus MoncloaCEI Campus Moncloa.

Animal Health and Veterinary Laboratories AgencyAnimal Health and Veterinary Laboratories Agency (AHVLA).

Instituto de Salud Carlos IIICentro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES). Instituto de Salud Carlos III (ISCIII).

Comunidad de MadridServicio de Microbiología y Enfermedades Infecciosas. Instituto de Investigación Sanitaria Gregorio Marañón (IISGM). Hospital General Universitario Gregorio Marañón. Consejería de Sanidad. Comunidad de Madrid.







Journal of Clinical Microbiology
FACTOR YEAR Q
4.232 2013

NLMID: 7505564

PMID: 23985914

ISSN: 0095-1137



TITLE: Splitting of a prevalent Mycobacterium bovis spoligotype by Variable Number Tandem Repeat Typing reveals high heterogeneity in an evolving clonal group


JOURNAL: J. Clin. Microbiol.


NUMERACIÓN: 51(11):3658-65


AÑO: 2013


PUBLISHER: American Society for Microbiology


AUTHORS: Rodriguez-Campos S., Navarro Y., Romero B., de Juan L., Bezos J., Mateos A., Golby P., Smith NH., Hewinson RG., Dominguez L., Garcia de Viedma D. and Aranaz A.


VISAVET PARTICIPANTS


3rd
Beatriz Romero Martínez
4th
Lucía de Juan Ferré
5th
Javier Bezos Garrido
10
Lucas Domínguez Rodríguez

DOI: https://doi.org/10.1128/JCM.01271-13


CITE THIS PUBLICATION:

Rodriguez-Campos S., Navarro Y., Romero B., de Juan L., Bezos J., Mateos A., Golby P., Smith NH., Hewinson RG., Dominguez L., Garcia de Viedma D. and Aranaz A. Splitting of a prevalent Mycobacterium bovis spoligotype by Variable Number Tandem Repeat Typing reveals high heterogeneity in an evolving clonal group. Journal of Clinical Microbiology. 51(11):3658-65. 2013. (A). ISSN: 0095-1137. DOI: 10.1128/JCM.01271-13


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