Contact
Microbial Identification and Characterisation Unit
VISAVET Health Surveillance Centre
Universidad Complutense
Av. Puerta de Hierro s/n. 28040 Madrid. Spain
e-mail:
Head office phone: (+34) 913 944 096
Laboratory phone: (+34) 913 944 002
Fax: (+34) 913 943 795
This unit is focused on the study at the phenotypic, genomic and protein leves of pathogenic bacteria of veterinary interest. The main activities of the Unit are divided into three research lines that are closely related: clinical diagnosis (using phenotypic, molecular and chemotaxonomic methods, the molecular characterization of pathogenic bacteria of relevant clinical significance for epidemiological purposes and description of new bacterial species (bacterial taxonomy). The molecular characterization allows tracing epidemiological links between strains associated with outbreaks of disease, global epidemiological studies, monitoring programs, etc. Studies of bacterial taxonomy not only allow the description of new species but also allow, for example, the association of different pathogens with atypical clinical processes. Additionally, this unit integrates the Mass Spectrometry VISAVET- Bruker Platform enabling developing proteomics research studies in the field of microbiology and molecular histology.
This platform was acquired in the 2011 call of the Equipment Acquisition and Scientific-Technical Infrastructure Adequacy (CAIMON) of Campus MONCLOA and enable the constitution of a service to enhance the research on campus.
The VISAVET-BRUKER service is made within the framework of a collaboration agreement between the Complutense University and Bruker Daltonik GMBH.
Application for identification and classification of bacteria and fungi. The identification is based on the comparison of the spectral fingerprints of the microorganism with those existing in the database (composed for more than 4000 entries). The technique is an accurate, simple, fast and reliable alternative for the identification of bacteria with traditional methods.
MALDI BIOTYPER analysis services (coming soon)
Application of MALDI TOF MS for the two dimensional acquisition of mass spectra from fresh tissue section. Image MALDI is ideal for the identification of protein markers and other small molecules in tissue sections.
IMAGE MALDI analysis services (coming soon)
System allowing the identification of proteins primarily by determining its peptide fingerprint. Enables Proteomics studies.
Servicios de Análisis por LC-MALDI (coming soon)
Investigation
Research lines:
Next scientific publications:
- Valenzuela-Palomo A., Bueno-Martinez E., Sanoguera-Miralles L., Lorca V., Fraile-Bethencourt E., Esteban-Sanchez A., Gomez S., Carvalho J., Allen J., Garcia-Alvarez A., Perez-Segura P., Dorling L., Easton DF., Devilee P., Vreeswijk MPG., de la Hoya M. and Velasco EA.. Splicing predictions, minigene analyses and ACMG/AMP clinical classification of 42 germline PALB2 splice-site variants. Journal of Pathology. In Press (A)
Last scientific publications:
- Barroso-Arevalo S., Barneto A., Ramos AM., Rivera B., Sanchez R., Sanchez-Morales I., Perez-Sancho M., Buendia A., Ferreras E., Ortiz-Menendez JC., Moreno I., Serres C., Vela C., Risalde MA., Dominguez L. and Sanchez-Vizcaino JM.. Large-scale study on virological and serological prevalence of SARS-CoV-2 in cats and dogs in Spain. Transboundary and Emerging Diseases, 1-16. 2021. (A)
- Liang P., Wang M., Gottschalk M., Vela AI., Estrada AA., Wang J., Du P., Luo M., Wu Z. and Zheng H.. Genomic and Pathogenic Investigations of Streptococcus suis Serotype 7 Population derived from a Human Patient and Pigs. Emerging microbes and infections, 1-41. 2021. (A)
- Polo-Vaquero C., Garcia-Seco T., Hernandez M., Fernandez V., Rodriguez-Lazaro D., Goyache J., Dominguez L. and Perez-Sancho M.. Evaluation of PCR assays for Campylobacter fetus detection and discrimination between C. fetus subspecies in bovine preputial wash samples. Theriogenology, 172:300-306. 2021. (A)
- Arnal JL., Fernandez A., Vela AI., Sanz C., Fernandez-Garayzabal JF. and Cid D.. Capsular type diversity of Mannheimia haemolytica determined by multiplex real-time PCR and indirect hemagglutination in clinical isolates from cattle, sheep, and goats in Spain. Veterinary Microbiology, 258:109121. 2021. (A)
- Cardoso-Toset F., Gomez-Laguna J., Gomez-Gascon L., Rodriguez-Gomez IM., Galan-Relaño A., Carrasco L., Tarradas C., Vela AI. and Luque I.. Histopathological and microbiological study of porcine lymphadenitis: contributions to diagnosis and control of the disease. Porcine Health Management, 6(1):36. 2020. (A)
Last PhD thesis supervised:
- Leydis Zamora Morales. Estudio taxonómico de bacterias relacionadas con el Síndrome del Alevín de la trucha. 2015. (Premio Laboratorios Ovejero)
- Verónica Sánchez del Rey. Estructura y diversidad de la población de Streptococcus suis presente en el ganado porcino, jabalí y conejo silvestre. 2015.
- Monica Aguado Urda. Caracterización y análisis funcional del genoma de Lactococcus garvieae. 2014.
- Verena Blume Serrano. Molecular characterization of Streptococcus suis (serotypes 2 and 9) isolated from swine in Spain. 2010.
- Nerea García Benzaquén. Caracterización fenotípica y genética de aislados de Pasteurela multocida obtenidas de ganado porcino. 2009.
Outreach
Last outreach publications:
- Perez-Sancho M., Vela AI., Garcia-Seco T., Gottschalk M., Dominguez L. and Fernandez-Garayzabal JF.. Assessment of MALDI-TOF MS as alternative tool for Streptococcus suis identification. Applications of STEM (Science, Technology, Engineering and Mathematics) tools in microbiology of infectious diseases. Ed. 1. 113-118, Frontiers. 2017.
- Bezos J., Cots M. and Valls JL.. La Bronquitis Infecciosa Aviar. Merial Laboratorios S.A.. 2016.
- Lopez-Campos G., Aguado-Urda M., Blanco MM., Gibello A., Cutuli MT., Lopez-Alonso V., Martin-Sanchez F. and Fernandez-Garayzabal JF.. Lactococcus garvieae: a small bacteria and a big data world. Health Information Science and System, 24;3:S5, Universidad Complutense. 2015.
- Barrera R., Cerda P., Dominguez L., Fernandez-Garayzabal JF., Galiana F., Gomez J., Hernandez A., Hernandez JM., Herrero M., Lopez A., Lopez J., Meseguer E., Molina JR., Palacios E., Portela S., Ruiz F., Santiago JM., Seiz R., Tejedor JL., Torrent F., Valles M., Vela AI. and Villarroel M.. Acuicultura en aguas continentales. Ministerio de Medio Ambiente y Medio Rural y Marino. 2011.
- Garcia N., Cebolla JA. and Lopez G.. Pasteurella multocida. Anaporc, 56:37-39, Asociacion Nacional de Porcicultura Científica. 2009.
Last communications:
- Perez-Sancho M.. Transporte y envío de muestras biológicas: empaquetado y documentación. Apoyo del auxiliar pecuario en la toma de muestras. 2021. (Online communication)
- Perez-Sancho M.. Tipos de muestras para diagnóstico y análisis laboratoriales. Apoyo del auxiliar pecuario en la toma de muestras. 2021. (Online communication)
- Garcia-Seco T.. Muestras ambientales en sanidad animal. Apoyo del auxiliar pecuario en la toma de muestras. 2021. (Online communication)
- Perez-Sancho M., Herranz M., Palau-Poveda CS., Polo-Vaquero C., Casamayor A., Dominguez L. and Garcia-Seco T.. Advances in the diagnosis of contagious agalactia:development of a Taqman Real-Time PCR for the detection of Mycoplasma capricolum capricolum. 6th Congress of the Europena Association of Veterinary Laboratory Diagnosticians (EAVLD). 2021. (Poster communication)
- Polo-Vaquero C., Garcia-Seco T., Casamayor A., Diez-Guerrier A., Fernandez V., Dominguez L. and Perez-Sancho M.. Desing of a real-time PCR targeting EF1-alpha-Tf1 gene (EFa1) for Tritrichomonas foetus identification on preputial bull samples. 6th Congress of the Europena Association of Veterinary Laboratory Diagnosticians (EAVLD). 2021. (Oral communication)
