Contact
Microbial Identification and Characterisation Unit
VISAVET Health Surveillance Centre
Universidad Complutense
Av. Puerta de Hierro s/n. 28040 Madrid. Spain
e-mail:
Head office phone: (+34) 913 944 096
Laboratory phone: (+34) 913 943 975
This unit is focused on the study at the phenotypic, genomic and protein leves of pathogenic bacteria of veterinary interest. The main activities of the Unit are divided into three research lines that are closely related: clinical diagnosis (using phenotypic, molecular and chemotaxonomic methods, the molecular characterization of pathogenic bacteria of relevant clinical significance for epidemiological purposes and description of new bacterial species (bacterial taxonomy). The molecular characterization allows tracing epidemiological links between strains associated with outbreaks of disease, global epidemiological studies, monitoring programs, etc. Studies of bacterial taxonomy not only allow the description of new species but also allow, for example, the association of different pathogens with atypical clinical processes. Additionally, this unit integrates the Mass Spectrometry VISAVET- Bruker Platform enabling developing proteomics research studies in the field of microbiology and molecular histology.
This platform was acquired in the 2011 call of the Equipment Acquisition and Scientific-Technical Infrastructure Adequacy (CAIMON) of Campus MONCLOA and enable the constitution of a service to enhance the research on campus.
The VISAVET-BRUKER service is made within the framework of a collaboration agreement between the Complutense University and Bruker Daltonik GMBH.
Application for identification and classification of bacteria and fungi. The identification is based on the comparison of the spectral fingerprints of the microorganism with those existing in the database (composed for more than 4000 entries). The technique is an accurate, simple, fast and reliable alternative for the identification of bacteria with traditional methods.
MALDI BIOTYPER analysis services (coming soon)
Application of MALDI TOF MS for the two dimensional acquisition of mass spectra from fresh tissue section. Image MALDI is ideal for the identification of protein markers and other small molecules in tissue sections.
IMAGE MALDI analysis services (coming soon)
System allowing the identification of proteins primarily by determining its peptide fingerprint. Enables Proteomics studies.
Servicios de Análisis por LC-MALDI (coming soon)
Investigation
Research lines:
Next scientific publications:
- Vela AI., Blanco MM., Colussi S., Kotzamanidis C., Prearo M., Altinok I., Acutis P., Volpatti D., Alba P., Feltrin F., Ianzano A., Dominguez L. and Fernandez-Garayzabal JF.. The association of Lactococcus petauri with lactococcosis is older than expected. Aquaculture (Amsterdam, Netherlands). In Press (A)
Last scientific publications:
- Vargas-Castro I., Crespo-Picazo JL., Fayos M., Jimenez-Martinez MA., Torre-Fuentes L., Alvarez J., Moura AE., Hernandez M., Buendia A., Barroso-Arevalo S., Garcia-Seco T., Perez-Sancho M., de Miguel MJ., Andres-Barranco S., Marco-Cabedo V., Penin-Villahoz G., Munoz PM., Dominguez L., Garcia-Parraga D. and Sanchez-Vizcaino JM.. New insights into the pathogenesis and transmission of Brucella pinnipedialis: systemic infection in two bottlenose dolphins ( Tursiops truncatus). Microbiology Spectrum, e0199723. 2023. (A)
- Smith P., Le Devendec L., Jouy E., Larvor E., Le Breton A., Picon-Camacho S., Zrncic S., Zupicic IG., Oraic D., Karatas S., Verner-Jeffreys D., Wokorac-Joseph A., Light E., van Essen-Zanbergen A., van Gelderen B., Voobergen-Laarman M., Haenen OLM., Veldman KT., Madsen L., Mouritsen KK., Smith-Svanevik C., Hakonsholm F., Vela AI., Garcia M., Florio D., Fioravanti M., Cortinovis L., Pretto T., Manfrin A. and Baron S.. Epidemiological cut-off values for Vibrio anguillarum MIC and disc diffusion data generated by standardised methods. Diseases of Aquatic Organisms, 155:109-123. 2023. (A)
- Ibrahim Khalil SM., Bulfon C., Galeotti M., Acutis PL., Altinok I., Kotzamanidis C., Vela AI., Fariano L., Prearo M., Colussi S. and Volpatti D.. Immune profiling of rainbow trout (Oncorhynchus mykiss) exposed to Lactococcus garvieae: Evidence in asymptomatic versus symptomatic or vaccinated fish. Journal of Fish Diseases, 46(7):731-741. 2023. (A)
- Stoppani N., Colussi S., Pastorino P., Prearo M., Sciuto S., Altinok I., Ozturk RC., Ture M., Vela AI., Blanco MM., Kotzamanidis C., Bitchava K., Malousi A., Fariano L., Volpatti D., Acutis PL. and Fernandez-Garayzabal JF.. 16S-23S rRNA Internal Transcribed Spacer Region ( ITS) Sequencing: A Potential Molecular Diagnostic Tool for Differentiating Lactococcus garvieae and Lactococcus petauri. Microorganisms, 11(5):1320. 2023. (A)
- Barroso-Arevalo S., Diaz-de Frutos M., Kosowska A., Perez-Sancho M., Dominguez L. and Sanchez-Vizcaino JM.. A useful tool for the safe diagnosis and control of the two main pandemics of the XXI century: COVID-19 and African Swine Fever disease. PLoS ONE, 18(3):e0282632. 2023. (A)
Last PhD thesis supervised:
- Coral Polo Vaquero. New approaches in the control of infectious fertility problems in extensive cattle. 2023.
- Leydis Zamora Morales. Estudio taxonómico de bacterias relacionadas con el Síndrome del Alevín de la trucha. 2015. (Premio Laboratorios Ovejero)
- Verónica Sánchez del Rey. Estructura y diversidad de la población de Streptococcus suis presente en el ganado porcino, jabalí y conejo silvestre. 2015.
- Monica Aguado Urda. Caracterización y análisis funcional del genoma de Lactococcus garvieae. 2014.
- Verena Blume Serrano. Molecular characterization of Streptococcus suis (serotypes 2 and 9) isolated from swine in Spain. 2010.
Outreach
Last outreach publications:
- Perez-Sancho M., Vela AI., Garcia-Seco T., Gottschalk M., Dominguez L. and Fernandez-Garayzabal JF.. Assessment of MALDI-TOF MS as alternative tool for Streptococcus suis identification. Applications of STEM (Science, Technology, Engineering and Mathematics) tools in microbiology of infectious diseases. Ed. 1. 113-118, Frontiers. 2017.
- Bezos J., Cots M. and Valls JL.. La Bronquitis Infecciosa Aviar. Merial Laboratorios S.A.. 2016.
- Lopez-Campos G., Aguado-Urda M., Blanco MM., Gibello A., Cutuli MT., Lopez-Alonso V., Martin-Sanchez F. and Fernandez-Garayzabal JF.. Lactococcus garvieae: a small bacteria and a big data world. Health Information Science and System, 24;3:S5, Universidad Complutense. 2015.
- Barrera R., Cerda P., Dominguez L., Fernandez-Garayzabal JF., Galiana F., Gomez J., Hernandez A., Hernandez JM., Herrero M., Lopez A., Lopez J., Meseguer E., Molina JR., Palacios E., Portela S., Ruiz F., Santiago JM., Seiz R., Tejedor JL., Torrent F., Valles M., Vela AI. and Villarroel M.. Acuicultura en aguas continentales. Ministerio de Medio Ambiente y Medio Rural y Marino. 2011.
- Garcia N., Cebolla JA. and Lopez G.. Pasteurella multocida. Anaporc, 56:37-39, Asociacion Nacional de Porcicultura Científica. 2009.
Last communications:
- Perez-Sancho M.. Muestreos no invasivos: ¿está la clave para la detección de patógenos y el análisis de su virulencia y resistencia a antibióticos fuera del animal?. XXVI Edición del Simposio Nacional AVEDILA. 2023. (Oral communication)
- Sánchez-Morales L., Cruz F., Garcia-Seco T., Porras N., Acurio Gutsche KL., Perez-Domingo A., Barroso-Arevalo S., Diaz-de Frutos M., Perez-Sancho M. and Dominguez L.. Parámetros biopatológicos evaluados en sangre y plasma de ratones k18-hACE2 desafiados con SARS-CoV-2. XXVI Edición del Simposio Nacional AVEDILA. 2023. (Poster communication)
- Perez-Sancho M.. Identificación y caracterización microbiana mediante espectrometría de masas. Centro VISAVET-UCM: cómo se trabaja en un laboratorio de alta seguridad biológica. XXIII Semana de la Ciencia Madri+d. 2023. (Oral communication)
- Buendia A., Perez-Sancho M., Vela AI., Fernandez-Garayzabal JF., Casamayor A., Garcia-Seco T. and Dominguez L.. Infección concurrente por Lactococcus garvieae y Lactococcus petauri en una piscifactoría de trucha arcoíris. Jornadas Vigilancia Sanitaria 2023: presente y futuro para 2030. 2023. (Poster communication)
- Calderón-Bernal JM., Diez-Guerrier A., Dominguez L., Fernandez-Garayzabal JF., Vela AI. and Cid M.. Pasteurella multocida asociada a la Enfermedad Respiratoria Bovina. Jornadas Vigilancia Sanitaria 2023: presente y futuro para 2030. 2023. (Poster communication)
