VISAVET | Microbial Identification and Characterisation Unit

Contact

Microbial Identification and Characterisation Unit
VISAVET Health Surveillance Centre
Universidad Complutense
Av. Puerta de Hierro s/n. 28040 Madrid. Spain

e-mail:

Head office phone: (+34) 913 944 096

Laboratory phone: (+34) 913 944 002

Fax: (+34) 913 943 795

This unit is focused on the study at the phenotypic, genomic and protein leves of pathogenic bacteria of veterinary interest. The main activities of the Unit are divided into three research lines that are closely related: clinical diagnosis (using phenotypic, molecular and chemotaxonomic methods, the molecular characterization of pathogenic bacteria of relevant clinical significance for epidemiological purposes and description of new bacterial species (bacterial taxonomy). The molecular characterization allows tracing epidemiological links between strains associated with outbreaks of disease, global epidemiological studies, monitoring programs, etc. Studies of bacterial taxonomy not only allow the description of new species but also allow, for example, the association of different pathogens with atypical clinical processes. Additionally, this unit integrates the Mass Spectrometry VISAVET- Bruker Platform enabling developing proteomics research studies in the field of microbiology and molecular histology.

VISAVET-BRUKER Mass Spectrometry

This platform was acquired in the 2011 call of the Equipment Acquisition and Scientific-Technical Infrastructure Adequacy (CAIMON) of Campus MONCLOA and enable the constitution of a service to enhance the research on campus.

The VISAVET-BRUKER service is made within the framework of a collaboration agreement between the Complutense University and Bruker Daltonik GMBH.

MALDI BIOTYPER system

Application for identification and classification of bacteria and fungi. The identification is based on the comparison of the spectral fingerprints of the microorganism with those existing in the database (composed for more than 4000 entries). The technique is an accurate, simple, fast and reliable alternative for the identification of bacteria with traditional methods.

MALDI BIOTYPER analysis services (coming soon)

IMAGE MALDI system

Application of MALDI TOF MS for the two dimensional acquisition of mass spectra from fresh tissue section. Image MALDI is ideal for the identification of protein markers and other small molecules in tissue sections.

IMAGE MALDI analysis services (coming soon)

LC-MALDI system

System allowing the identification of proteins primarily by determining its peptide fingerprint. Enables Proteomics studies.

Servicios de Análisis por LC-MALDI (coming soon)

Investigation

Research lines:

Application of MALDI-TOF technique in the clinical microbiology and Agri-food field (ICM)
Clinical diagnosis (ICM)
Molecular typing (ICM)
Bacterial taxonomy (ICM)

Next scientific publications:

Gibello A., Vela AI., Martinez-Nevado E., Rodriguez-Bertos A., Casamayor A., Garcia J., Dominguez L., Montoto P., Fernandez-Garayzabal JF. and Amaro C.. Potentially human-virulent Vibrio vulnificus isolates from diseased great pompano (Trachinotus goodei).. Transboundary and Emerging Diseases. In Press (A)

Last scientific publications:

Castro R., Coll J., Blanco MDM., Rodriguez-Bertos A., Jouneau L., Fernandez-Garayzabal JF. and Gibello A.. Spleen and head kidney differential gene expression patterns in trout infected with Lactococcus garvieae correlate with spleen granulomas. Veterinary Research, 50(1):32. 2019. (A)
Galan-Relaño A., Gomez-Gascon L., Luque I., Barrero-Dominguez B., Casamayor A., Cardoso-Toset F., Vela AI., Fernandez-Garayzabal JF. and Tarradas C.. Antimicrobial susceptibility and genetic characterization of Trueperella pyogenes isolates from pigs reared under intensive and extensive farming practices. Veterinary Microbiology, 232:89-95. 2019. (A)
Cid D., Garcia-Alvarez A., Dominguez L., Fernandez-Garayzabal JF. and Vela AI.. Pasteurella multocida isolates associated with ovine pneumonia are toxigenic. Veterinary Microbiology, 232:40_73. 2019. (A)
Perez-Sancho M., Vela AI., Horcajo P., Ugarte-Ruiz M., Dominguez L., Fernandez-Garayzabal JF. and de la Fuente R.. Rapid differentiation of Staphylococcus aureus subspecies based on MALDI-TOF MS profiles. Journal of Veterinary Diagnostic Investigation , 30(6):813-820. 2018. (A)
Perez-Sancho M., Cerda I., Fernandez-Bravo A., Dominguez L., Figueras MJ., Fernandez-Garayzabal JF. and Vela AI.. Limited performance of MALDI-TOF for identification of fish Aeromonas isolates at species level. Journal of Fish Diseases, 41(10):1485-1493. 2018. (A)

See all scientific publications

Last PhD thesis supervised:

Leydis Zamora Morales. Estudio taxonómico de bacterias relacionadas con el Síndrome del Alevín de la trucha. 2015. (Premio Laboratorios Ovejero)
Verónica Sánchez del Rey. Estructura y diversidad de la población de Streptococcus suis presente en el ganado porcino, jabalí y conejo silvestre. 2015.
Monica Aguado Urda. Caracterización y análisis funcional del genoma de Lactococcus garvieae. 2014.
Verena Blume Serrano. Molecular characterization of Streptococcus suis (serotypes 2 and 9) isolated from swine in Spain. 2010.
Nerea García Benzaquén. Caracterización fenotípica y genética de aislados de Pasteurela multocida obtenidas de ganado porcino. 2009.

See all thesis

Outreach

Last outreach publications:

Perez-Sancho M., Vela AI., Garcia-Seco T., Gottschalk M., Dominguez L. and Fernandez-Garayzabal JF.. Assessment of MALDI-TOF MS as alternative tool for Streptococcus suis identification. Applications of STEM (Science, Technology, Engineering and Mathematics) tools in microbiology of infectious diseases. Ed. 1. 113-118, Frontiers. 2017.
Bezos J., Cots M. and Valls JL.. La Bronquitis Infecciosa Aviar. Merial Laboratorios S.A.. 2016.
Lopez-Campos G., Aguado-Urda M., Blanco MM., Gibello A., Cutuli MT., Lopez-Alonso V., Martin-Sanchez F. and Fernandez-Garayzabal JF.. Lactococcus garvieae: a small bacteria and a big data world. Health Information Science and System, 24;3:S5, Universidad Complutense. 2015.
Barrera R., Cerda P., Dominguez L., Fernandez-Garayzabal JF., Galiana F., Gomez J., Hernandez A., Hernandez JM., Herrero M., Lopez A., Lopez J., Meseguer E., Molina JR., Palacios E., Portela S., Ruiz F., Santiago JM., Seiz R., Tejedor JL., Torrent F., Valles M., Vela AI. and Villarroel M.. Acuicultura en aguas continentales. Ministerio de Medio Ambiente y Medio Rural y Marino. 2011.
Garcia N., Cebolla JA. and Lopez G.. Pasteurella multocida. Anaporc, 56:37-39, Asociacion Nacional de Porcicultura Científica. 2009.