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Application of the Enfer chemiluminescent multiplex ELISA system for the detection of Mycobacterium bovis infection in goats

Veterinary Microbiology publica este artículo de investigación

27 de enero de 2012

A study was conducted to optimise a multiplex serological immunoassay for use in identification of goats infected with Mycobacterium bovis. To assess assay specificity, 31 goats with a history of being free from M. bovis infection were used. To determine assay sensitivity, 180 Single Intradermal Comparative Tuberculin test (SICTT) positive goats were recruited. Additionally, 286 SICTT negative goats classed as potentially exposed animals present in the same positive herds were also included in the study. The results of the assay demonstrated a specificity of 100%. The multiplex assay detected 57/60 SICTT (95.0%) positive animals in one M. bovis infected herd and 120/120 (100%) in a second herd. In a separate experiment, 28 M. caprae culture confirmed infected goats from Spain were assayed, of which 24 (85.7%) were found positive in the test. The results show that inclusion of an antibody based assay can improve the ability to identify M. bovis and M. caprae infected goats. With further development and validation the multiplex assay may prove to be a useful tool for control of M. bovis and M. caprae infection in goats




Shuralev E., Quinn P., Doyle M., Duignan A., Kwok HF., Bezos J., Olwill SA., Gormley E., Aranaz A., Good M., Davis WC., Clarke J. y Whelan C.




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Application of the Enfer chemiluminescent multiplex ELISA system for the detection of Mycobacterium bovis infection in goats

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Application of the Enfer chemiluminescent multiplex ELISA system for the detection of Mycobacterium bovis infection in goats


Participantes:

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Enfer Scientific.

University College DublinUCD School of Agriculture, Food Science & Veterinary Medicine. University College Dublin (UCD).

Department of Agriculture, Fisheries and Food (DAFF).

Fusion Antibodies Ltd.

Department of Veterinary Microbiology and Pathology. College of Veterinary Medicine. Washington State University (WSU).







Veterinary Microbiology
FACTOR YEAR Q
3.127 2012

NLMID: 7705469

PMID: 21862242

ISSN: 0378-1135



TÍTULO: Application of the Enfer chemiluminescent multiplex ELISA system for the detection of Mycobacterium bovis infection in goats


REVISTA: Vet. Microbiol.


NUMERACIÓN: 154(3-4):292-7


AÑO: 2012


EDITORIAL: Elsevier Science Publishers


AUTORES: Shuralev E., Quinn P., Doyle M., Duignan A., Kwok HF., Bezos J., Olwill SA., Gormley E., Aranaz A., Good M., Davis WC., Clarke J. and Whelan C.


PARTICIPANTES VISAVET


Javier Bezos Garrido

DOI: https://doi.org/10.1016/j.vetmic.2011.07.028


CITA ESTA PUBLICACIÓN:

Shuralev E., Quinn P., Doyle M., Duignan A., Kwok HF., Bezos J., Olwill SA., Gormley E., Aranaz A., Good M., Davis WC., Clarke J. y Whelan C. Application of the Enfer chemiluminescent multiplex ELISA system for the detection of Mycobacterium bovis infection in goats. Veterinary Microbiology. 154(3-4):292-7. 2012. (A). ISSN: 0378-1135. DOI: 10.1016/j.vetmic.2011.07.028


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