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Assessment of different selective agar media for enumeration and isolation of Listeria from dairy products

Investigación publicada en The Journal of Dairy Research

1 de noviembre de 1988

Different selective agar media were compared for the recovery and isolation of five species of Listeria from raw milk and cheese. The selective media examined were Beerens medium, MacBride medium and that described by Dominguez et al. (1984) with 6 mg/l acriflavine, listeria selective agar medium (LSAM), and LSAM with 12 mg/l acriflavine (LSAM X 2A); a non-selective yeast glucose Lemco agar was included for comparison. When the difference between listeria and the natural microflora of raw milk and cheese was 10(2) cfu/ml, listeria could be isolated by direct plating on all media tested. When it was lower than 10(3)-10(4) cfu/ml, listeria were isolated by direct plating only on LSAM and LSAM X 2A. When the difference was greater than 10(4) cfu/ml, a previous enrichment was necessary to isolate them. LSAM and LSAM X 2A media performed better than the other media tested for isolating listeria by direct plating and improved their isolation from dairy products. This superior performance was evaluated by the ability of these media to support colony formation of different species of Listeria tested, the easy recognition of these colonies from those formed by other microorganisms and by their capacity to inhibit the natural microflora of these foods




Dominguez L., Fernandez-Garayzabal JF., Briones V., Blanco JL. y Suarez G.




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Assessment of different selective agar media for enumeration and isolation of Listeria from dairy products

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Assessment of different selective agar media for enumeration and isolation of Listeria from dairy products



Participantes:

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Servicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).







FACTOR YEAR Q
1.620 2005

NLMID: 2985125R

PMID: 3235719

ISSN: 0022-0299



TÍTULO: Assessment of different selective agar media for enumeration and isolation of Listeria from dairy products


REVISTA: J Dairy Res


NUMERACIÓN: 55(4):579-83


AÑO: 1988


EDITORIAL: Cambridge University Press


AUTORES: Dominguez L., Fernandez-Garayzabal JF., Briones V., Blanco JL. and Suarez G.


First
Lucas Domínguez Rodríguez
2nd
José Francisco Fernández-Garayzabal Fernández
3rd
Víctor Briones Dieste
4th
José Luis Blanco Cancelo

DOI: https://doi.org/10.1017/S0022029900033367


CITA ESTA PUBLICACIÓN:

Dominguez L., Fernandez-Garayzabal JF., Briones V., Blanco JL. y Suarez G. Assessment of different selective agar media for enumeration and isolation of Listeria from dairy products. The Journal of Dairy Research. 55(4):579-83. 1988. (A). ISSN: 0022-0299. DOI: 10.1017/S0022029900033367


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