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Characterization of Leishmania infantum strains by PCR-RFLP in wild Leporidae from the Community of Madrid


19 de mayo de 2017

Ortega-García MV., Moreno I., Garcia N., Martin AB., Navarro A., Martinez I., Garcia-Seco T., Perez-Sancho M. y Dominguez M.

Comunicación presentada en 6th World Congress on Leishmaniasis



Leishmania infantum is the main species implicated in Leishmaniosis in the Mediterranean basin, where the disease is endemic. Canines are the main domestic reservoirs. Recently, Leporidae have been revealed as competent reservoirs of L. infantum, being the source of infection during an important outbreak in Madrid (Spain) that involved 697 human cases from 2009 to 2016. L. infantun DNA has been detected by quantitative real-time PCR in wild rabbits and hares in two Madrid natural areas outside the outbreak area. Comparison of the strains isolated from different areas could add more knowledge about the sylvatic cycle of Leishmania spp. and PCR-RFLP can be used as a cost-effective and reliable method to achieve it.
The aims of this study are a) to characterize the L. infantum strains detected in wild Leporidae from different areas of the Community of Madrid (outbreak and non-outbreak areas); and b), to know if the L. infantum strains maintained by wild Leporidae in the outbreak area are the same as those ones found in the non-outbreak areas



Participantes:

Ministerio de DefensaCampus La Marañosa (ITM). Instituto Nacional de Técnica Aeroespacial (INTA). Ministerio de Defensa.

Ministerio de DefensaSubdirección de Sistemas Terrestres. Instituto Nacional de Técnica Aeroespacial (INTA). Ministerio de Defensa.

Instituto de Salud Carlos IIIServicio de Inmunología. Centro Nacional de Microbiología (CNM). Instituto de Salud Carlos III (ISCIII).

Universidad ComplutenseServicio de Zoonosis Emergentes, de Baja Prevalencia y Agresivos Biológicos (NED). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).


Enlace a 6th World Congress on Leishmaniasis





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6th World Congress on Leishmaniasis


6th World Congress on Leishmaniasis
16-20 mayo de 2017
Toledo
España

TÍTULO: Characterization of Leishmania infantum strains by PCR-RFLP in wild Leporidae from the Community of Madrid


TIPO: Comunicación en póster


AUTORES: Ortega-García MV., Moreno I., Garcia N., Martin AB., Navarro A., Martinez I., Garcia-Seco T., Perez-Sancho M. y Dominguez M.


PARTICIPANTES VISAVET


First
Mª Victoria Ortega García
3rd
Nerea García Benzaquén
5th
Alejandro Navarro Gómez
6th
Irene Martínez Alares
7th
Teresa García-Seco Romero
8th
Marta Pérez Sancho

FECHA: 19 de mayo de 2017



CITA ESTA COMUNICACIÓN:

Ortega-García MV., Moreno I., Garcia N., Martin AB., Navarro A., Martinez I., Garcia-Seco T., Perez-Sancho M. y Dominguez M. Characterization of Leishmania infantum strains by PCR-RFLP in wild Leporidae from the Community of Madrid. 6th World Congress on Leishmaniasis, Instituto de Salud Carlos III, Drugs for Neglected Diseases initiative, Toledo, España, 19 de mayo de 2017.


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