Chryseobacterium oncorhynchi sp. nov., isolated from rainbow trout (Oncorhynchus mykiss)
Investigación publicada en Systematic and Applied Microbiology
1 de febrero de 2012
Genotypic and phenotypic analyses were performed on five Gram-negative, catalase and oxidasepositive, rod-shaped bacteria isolated from the gill and liver of four rainbow trout. Studies based on comparative 16S rRNA gene sequence analysis showed that the five new isolates shared 99.8–100% sequence similarity and that they belong to the genus Chryseobacterium. The nearest phylogenetic neighbours of the strain 701B-08T were Chryseobacterium ureilyticum F-Fue-04IIIaaaaT (99.1% 16S rRNA gene sequence similarity) and Chryseobacterium joosteii LMG 18212T (98.6%). DNA–DNA hybridization values between the five isolates were 91–99% and ranged from 2 to 53% between strain 701B-08T and the type strains of phylogenetically closely related species of Chryseobacterium. Strain 701B-08T had a DNA G + C content of 36.3 mol%, the major fatty acids were iso-C15:0, iso-C17:1 ω9c, C16:1 ω6c and iso-C17:0 3-OH and the predominant respiratory quinone was MK-6. The novel isolates were distinguished from related Chryseobacterium species by physiological and biochemical tests. The genotypic and phenotypic properties of the isolates from rainbow trout suggest their classification as representatives of a novel species of the genus Chryseobacterium, for which the name Chryseobacterium oncorhynchi sp. nov. is proposed. The type strain is 701B-08T (=CECT 7794T = CCUG 60105T).
Zamora L., Fernandez-Garayzabal JF., Palacios MA., Sanchez-Porro C., Svensson-Stadler LA., Dominguez L., Moore ERB., Ventosa A. y Vela AI..
Servicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM). | |
Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM). | |
Piscifactoría Zorita Illana, S.A. (PISZOLLA). | |
Departamento de Microbiología y Parasitología. Facultad de Farmacia. Universidad de Sevilla (US). | |
Sahlgrenska Academy. University of Gothenburg. | |