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Day-old chicks are a source of antimicrobial resistant bacteria for laying hen farms

Investigación publicada en Veterinary Microbiology

1 de marzo de 2019

Antimicrobial resistant bacteria are rarely detected in laying hens and the objective of this longitudinal study was to test day-old chick as a source. Four different commercial batches raised on the same farm were monitored from day-old chick to laying hens using Escherichia coli as a model. Ten colonies from each of the eight samplings per batch were tested for antimicrobial susceptibility using 14 antimicrobials.
Overall (313 isolates), higher resistance percentages were detected for tetracycline (26.8%), followed by sulphonamides (16.3%), ampicillin (16.0 %) and quinolones (10.9% and 9.3% for ciprofloxacin and nalidixic acid, respectively). Resistance percentages of bacteria from day-old chicks were higher than those of pullets and hens (p < 0.05) for tetracycline, sulphonamides, trimethoprim and chloramphenicol.
Forty different phenotypic resistance profiles were detected, led by fully susceptible (182 isolates; 58.1%), and followed by single tetracycline (28 isolates; 8.9%) and ciprofloxacin/ nalidixic acid (11 isolates; 3.5%) profiles.
By whole-genome sequencing, 17 genes and mutations of five chromosomal genes related to resistance were detected, the most frequent being tetA, blaTEM-1B and sul1.
Using multilocus sequencing analysis, 58 different MLST types were detected, most of them only in a particular sample. The ST155 (27/142) was the most frequently detected, followed by ST10 (19/142) and ST48 (9/142).
The fate on the farm of the detected E. coli populations in old-day chicks was not clear, but our data suggest that they did not remain in the predominant faecal population of pullets and laying hens




Moreno MA., Garcia-Soto S., Hernandez M., Barcena C., Rodriguez-Lazaro D., Ugarte-Ruiz M. y Dominguez L..




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Day-old chicks are a source of antimicrobial resistant bacteria for laying hen farms

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Day-old chicks are a source of antimicrobial resistant bacteria for laying hen farms

Participantes:

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Instituto Tecnológico Agrario de Castilla y LeónLaboratorio de Biología Molecular y Microbiología. Instituto Tecnológico Agrario de Castilla y León (ITACyL).

Universidad de BurgosUnidad de Microbiología. Departamento de Biotecnología y Ciencia de los Alimentos. Universidad de Burgos (UBU).







Veterinary Microbiology
FACTOR YEAR Q
2.524 2017

NLMID: 7705469

PMID: 30827391

ISSN: 0378-1135



TÍTULO: Day-old chicks are a source of antimicrobial resistant bacteria for laying hen farms


REVISTA: Vet. Microbiol.


NUMERACIÓN: 230:221-227


AÑO: 2019


EDITORIAL: Elsevier Science Publishers


AUTORES: Moreno MA., Garcia-Soto S., Hernandez M., Barcena C., Rodriguez-Lazaro D., Ugarte-Ruiz M. and Dominguez L..


PARTICIPANTES VISAVET


First
Miguel Ángel Moreno Romo
4th
Carmen Bárcena Asensio
6th
María Ugarte Ruiz
Last
Lucas Domínguez Rodríguez

DOI: https://doi.org/10.1016/j.vetmic.2019.02.007


CITA ESTA PUBLICACIÓN:

Moreno MA., Garcia-Soto S., Hernandez M., Barcena C., Rodriguez-Lazaro D., Ugarte-Ruiz M. y Dominguez L. Day-old chicks are a source of antimicrobial resistant bacteria for laying hen farms. Veterinary Microbiology. 230:221-227. 2019. (A). ISSN: 0378-1135. DOI: 10.1016/j.vetmic.2019.02.007


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