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Evaluation of the immunogenicity and safety of Brucella melitensis B115 vaccination in pregnant sheep

Vaccine publica este artículo de investigación

1 de abril de 2014

In spite of its limitations, Rev.1 is currently recognized as the most suitable vaccine against Brucella melitensis (the causative agent of ovine and caprine brucellosis). However, its use is limited to young animals when test-and-slaughter programs are in place because of the occurrence of false positive-reactions due to Rev.1 vaccination. The B. melitensis B115 rough strain has demonstrated its
efficacy against challenge with B. melitensis virulent strains in the mouse model, but there is a lack of information regarding its potential use in small ruminants for brucellosis control. Here, the safety and immune response elicited by B115 strain inoculation were evaluated in pregnant ewes vaccinated at their midpregnancy. Vaccinated (n=8) and non-vaccinated (n=3) sheep were periodically sampled and analyzed on the 108 days following inoculation using tests designed for detection of the response elicited by the B115 strain and routine serological tests for brucellosis [Rose Bengal Test (RBT),Complement Fixation Test (CFT) and blocking ELISA (ELISAb)]. Five out of the 8 vaccinated animals aborted, indicating a significant abortifacient effect of B115 inoculation at midpregnancy. In addition, a smooth strain was recovered from one vaccinated animal, suggesting the occurrence of an in-vivo reversion phenomenon. Only one animal was positive in both RBT and CFT simultaneously (91 days after vaccination) confirming the lack of induction of cross-reacting antibody responses interfering with routine brucellosis diagnostic tests in most B115-vaccinated animals




Perez-Sancho M., Adone R., Garcia-Seco T., Tarantino M., Diez-Guerrier A., Drumo R., Francia M., Dominguez L., Pasquali P. y Alvarez J..




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Evaluation of the immunogenicity and safety of Brucella melitensis B115 vaccination in pregnant sheep

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Evaluation of the immunogenicity and safety of Brucella melitensis B115 vaccination in pregnant sheep


Participantes:

Universidad ComplutenseServicio de Zoonosis Emergentes, de Baja Prevalencia y Agresivos Biológicos (NED). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Istituto Superiore di SanitàIstituto Superiore di Sanità.

Comunidad de MadridInstituto Ramón y Cajal de Investigación Sanitaria (IRYCIS). Universidad Complutense (UCM). Universidad Autónoma de Madrid (UAM). Universidad de Alcalá (UAH). Salud Madrid. Comunidad de Madrid.







Vaccine
FACTOR YEAR Q
3.624 2014

NLMID: 8406899

PMID: 24508034

ISSN: 0264-410X



TÍTULO: Evaluation of the immunogenicity and safety of Brucella melitensis B115 vaccination in pregnant sheep


REVISTA: Vaccine


NUMERACIÓN: 32 (2014):1877–1881


AÑO: 2014


EDITORIAL: Elsevier Science


AUTORES: Perez-Sancho M., Adone R., Garcia-Seco T., Tarantino M., Diez-Guerrier A., Drumo R., Francia M., Dominguez L., Pasquali P. and Alvarez J..


PARTICIPANTES VISAVET


First
Marta Pérez Sancho
3rd
Teresa García-Seco Romero
5th
Alberto Antoine Díez Guerrier
8th
Lucas Domínguez Rodríguez
Last
Julio Álvarez Sánchez

DOI: https://doi.org/10.1016/j.vaccine.2014.01.070


CITA ESTA PUBLICACIÓN:

Perez-Sancho M., Adone R., Garcia-Seco T., Tarantino M., Diez-Guerrier A., Drumo R., Francia M., Dominguez L., Pasquali P. y Alvarez J. Evaluation of the immunogenicity and safety of Brucella melitensis B115 vaccination in pregnant sheep. Vaccine. 32 (2014):1877–1881. 2014. (A). ISSN: 0264-410X. DOI: 10.1016/j.vaccine.2014.01.070


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