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Flavobacterium plurextorum sp. nov. isolated from farmed rainbow trout (Oncorhynchus mykiss)

Artículo de investigación publicado en PLoS ONE

25 de junio de 2013

Five strains (1126-1H-08T, 51B-09, 986-08, 1084B-08 and 424-08) were isolated from diseased rainbow trout. Cells were Gram-negative rods, 0.7 µm wide and 3 µm long, non-endospore-forming, catalase and oxidase positive. Colonies were circular, yellow-pigmented, smooth and entire on TGE agar after 72 hours incubation at 25°C. They grew in a temperature range between 15°C to 30°C, but they did not grow at 37°Cor 42°C. Based on 16S rRNA gene sequence analysis, the isolates belonged to the genus Flavobacterium. Strain 1126-1H-08T exhibited the highest levels of similarity with Flavobacterium oncorhynchi CECT 7678T and Flavobacterium pectinovorum DSM 6368T (98.5% and 97.9% sequence similarity, respectively). DNA–DNA hybridization values were 87 to 99% among the five isolates and ranged from 21 to 48% between strain 1126-1H-08T, selected as a representative isolate, and the type strains of Flavobacterium oncorhynchi CECT 7678T and other phylogenetic related Flavobacterium species. The DNA G+C content of strain 1126-1H-08T was 33.2 mol%. The predominant respiratory quinone was MK-6 and the major fatty acids were iso-C15:0 and C15:0. These data were similar to those reported for Flavobacterium species. Several physiological and biochemical tests differentiated the novel bacterial strains from related Flavobacterium species. Phylogenetic, genetic and phenotypic data indicate that these strains represent a new species of the genus Flavobacterium, for which the name Flavobacterium plurextorum sp. nov. was proposed. The type strain is 1126-1H-08T ( = CECT 7844T = CCUG 60112T)




Zamora L., Fernandez-Garayzabal JF., Sanchez-Porro C., Palacios MA., Moore ERB., Dominguez L., Ventosa A. y Vela AI..




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Flavobacterium plurextorum sp. nov. isolated from farmed rainbow trout (Oncorhynchus mykiss)

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Flavobacterium plurextorum sp. nov. isolated from farmed rainbow trout (Oncorhynchus mykiss)


Participantes:

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Piscifactoría Zorita Illana, S.A. (PISZOLLA).

Universidad de SevillaFacultad de Farmacia. Universidad de Sevilla (US).

Sahlgrenska Academy. University of Gothenburg.

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







PLoS ONE
FACTOR YEAR Q
3.534 2013

NLMID: 101285081

PMID: 23825681

ISSN: 1932-6203



TÍTULO: Flavobacterium plurextorum sp. nov. isolated from farmed rainbow trout (Oncorhynchus mykiss)


REVISTA: PLoS ONE


NUMERACIÓN: 8(6):e67741


AÑO: 2013


EDITORIAL: Public Library Science


AUTORES: Zamora L., Fernandez-Garayzabal JF., Sanchez-Porro C., Palacios MA., Moore ERB., Dominguez L., Ventosa A. and Vela AI..


PARTICIPANTES VISAVET


2nd
José Francisco Fernández-Garayzabal Fernández
6th
Lucas Domínguez Rodríguez
Last
Ana Isabel Vela Alonso

DOI: https://doi.org/10.1371/journal.pone.0067741


CITA ESTA PUBLICACIÓN:

Zamora L., Fernandez-Garayzabal JF., Sanchez-Porro C., Palacios MA., Moore ERB., Dominguez L., Ventosa A. y Vela AI. Flavobacterium plurextorum sp. nov. isolated from farmed rainbow trout (Oncorhynchus mykiss). PLoS ONE. 8(6):e67741. 2013. (A). ISSN: 1932-6203. DOI: 10.1371/journal.pone.0067741


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