Comunicación presentada en 6th Congress of European Microbiologists

7 de junio de 2015

Perez-Sancho M., Liandris E., Casamayor A., Pulido E. y Dominguez L.

Although direct smear technique is an excellent approach for the preparation of
samples for MALDI-TOF MS identification, the usefulness of this approach is limited
to BSL-1/BSL-2 bacteria, it is not recommended for MSP creation and it requires
fresh material for an optimal performance. Formic acid extraction is proposed as a
more precise method for MALDI Biotyper identification that includes a step in which
bacteria are suspended in 70% ethanol which could potentially be used for the
storage of bacterial suspensions in their optimal growth phase. However, there is a
lack of information regarding the impact of storage of bacterial suspensions in ethanol on the the reliability of the MALDI-Biotyper identification

	CEI Campus Moncloa.
	Servicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

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