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MALDI-TOF Mass Spectrometry as a Rapid Screening Alternative for Non-tuberculous Mycobacterial Species Identification in the Veterinary Laboratory

Frontiers in veterinary science publica este artículo de investigación

28 de enero de 2022

Non-tuberculous mycobacteria (NTM) are difficult to identify by biochemical and genetic methods due to their microbiological properties and complex taxonomy. The development of more efficient and rapid methods for species identification in the veterinary microbiological laboratory is, therefore, of great importance. Although MALDI-TOF Mass Spectrometry (MS) has become a promising tool for the identification of NTM species in human clinical practise, information regarding its performance on veterinary isolates is scarce. This study assesses the capacity of MALDI-TOF MS to identify NTM isolates (n = 75) obtained from different animal species. MALDI-TOF MS identified 76.0% (n = 57) and 4% (n = 3) of the isolates with high and low confidence, respectively, in agreement with the identification achieved by Sanger sequencing of housekeeping genes (16S rRNA, hsp65, and rpoB). Thirteen isolates (17.3%) were identified by Sanger sequencing to the complex level, indicating that these may belong to uncharacterised species. MALDI-TOF MS approximated low confidence identifications toward closely related mycobacterial groups, such as the M. avium or M. terrae complexes. Two isolates were misidentified due to a high similarity between species or due to the lack of spectra in the database. Our results suggest that MALDI-TOF MS can be used as an effective alternative for rapid screening of mycobacterial isolates in the veterinary laboratory and potentially for the detection of new NTM species. In turn, Sanger sequencing could be implemented as an additional method to improve identifications in species for which MALDI-TOF MS identification is limited or for further characterisation of NTM species




Lorente-Leal V., Liandris E., Bezos J., Perez-Sancho M., Romero B. y de Juan L..




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MALDI-TOF Mass Spectrometry as a Rapid Screening Alternative for Non-tuberculous Mycobacterial Species Identification in the Veterinary Laboratory

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MALDI-TOF Mass Spectrometry as a Rapid Screening Alternative for Non-tuberculous Mycobacterial Species Identification in the Veterinary Laboratory



Participantes:

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







Frontiers in veterinary science
FACTOR YEAR Q
3.471 2021

NLMID: 101666658

PMID: 35155660

ISSN: 2297-1769



TÍTULO: MALDI-TOF Mass Spectrometry as a Rapid Screening Alternative for Non-tuberculous Mycobacterial Species Identification in the Veterinary Laboratory


REVISTA: Front Vet Sci


NUMERACIÓN: 9:827702


AÑO: 2022


EDITORIAL: Lausanne : Frontiers Media S.A


AUTORES: Lorente-Leal V., Liandris E., Bezos J., Perez-Sancho M., Romero B. and de Juan L..


PARTICIPANTES VISAVET


First
Víctor Lorente Leal
3rd
Javier Bezos Garrido
4th
Marta Pérez Sancho
5th
Beatriz Romero Martínez
Last
Lucía de Juan Ferré

DOI: https://doi.org/10.3389/fvets.2022.827702


CITA ESTA PUBLICACIÓN:

Lorente-Leal V., Liandris E., Bezos J., Perez-Sancho M., Romero B. y de Juan L. MALDI-TOF Mass Spectrometry as a Rapid Screening Alternative for Non-tuberculous Mycobacterial Species Identification in the Veterinary Laboratory. Frontiers in veterinary science. 9:827702. 2022. (A). ISSN: 2297-1769. DOI: 10.3389/fvets.2022.827702


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