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Microplate technique to determine hemolytic activity for routine typing of Listeria strains

Artículo de investigación publicado en Journal of Clinical Microbiology

1 de julio de 1986

Because the hemolysis produced by Listeria monocytogenes and Listeria seeligeri on blood agar is frequently difficult to interpret, we developed a microplate technique for the routine determination of hemolytic activity with erythrocyte suspensions. This microtechnique is a simple and reliable test for distinguishing clearly between hemolytic and nonhemolytic strains and could be used instead of the CAMP (Christie-Atkins-Munch-Petersen) test with Staphylococcus aureus in the routine typing of Listeria strains. Furthermore, our results suggest that the quantitation of the hemolytic activity of the Listeria strains, along with the D-xylose, L-rhamnose, and alpha-methyl-D-mannoside acidification tests, allows the differentiation of L. monocytogenes, L. seeligeri, and Listeria ivanovii. We also observed that the treatment of erythrocytes with crude exosubstances of rhodococcus equi, Pseudomonas fluorescens, Acinetobacter calcoaceticus, and S. aureus enhanced the hemolytic activity of all Listeria strains with this characteristi




Dominguez L., Vazquez-Boland JA., Fernandez-Garayzabal JF., Echalecu P., Gomez-Lucia E., Rodriguez-Ferri E. y Suarez G.




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Microplate technique to determine hemolytic activity for routine typing of Listeria strains

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Microplate technique to determine hemolytic activity for routine typing of Listeria strains



Participantes:

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).







Journal of Clinical Microbiology
FACTOR YEAR Q
3.537 2005

NLMID: 7505564

PMID: 3088037

ISSN: 0095-1137



TÍTULO: Microplate technique to determine hemolytic activity for routine typing of Listeria strains


REVISTA: J Clin Microbiol


NUMERACIÓN: 24(1):99-103


AÑO: 1986


EDITORIAL: American Society for Microbiology


AUTORES: Dominguez L., Vazquez-Boland JA., Fernandez-Garayzabal JF., Echalecu P., Gomez-Lucia E., Rodriguez-Ferri E. and Suarez G.


First
Lucas Domínguez Rodríguez
3rd
José Francisco Fernández-Garayzabal Fernández

DOI: https://doi.org/10.1128/JCM.24.1.99-103.1986


CITA ESTA PUBLICACIÓN:

Dominguez L., Vazquez-Boland JA., Fernandez-Garayzabal JF., Echalecu P., Gomez-Lucia E., Rodriguez-Ferri E. y Suarez G. Microplate technique to determine hemolytic activity for routine typing of Listeria strains. Journal of Clinical Microbiology. 24(1):99-103. 1986. (A). ISSN: 0095-1137. DOI: 10.1128/JCM.24.1.99-103.1986


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