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Molecular characterization and antimicrobial resistance of STEC strains isolated from healthy cattle in 2011 and 2013 in Spain

Epidemiology and Infection publica este artículo de investigación

8 de julio de 2016

Prevention of Shiga toxin-producing Escherichia coli (STEC) foodborne outbreaks is hampered by its complex epidemiology. We assessed the distribution of virulence genes (VGs), main serogroups/serotypes for public health [haemolytic uraemic syndrome (HUS)-related], antimicrobial resistance (AMR) profiles and pulsed-field gel electrophoresis (PFGE) patterns in a collection of STEC isolates obtained from cattle hide (n = 149) and faecal (n = 406) samples collected during a national survey conducted in Spain in 2011 and 2013. Isolates were cultured using McConkey and CT-SMAC agar after enrichment, and confirmed as STEC by PCR. STEC prevalence in hides (15·4%) was higher than in faeces (10·7%) and O157:H7 was more frequent in the former (2·7% vs. 0·99%). Non-O157 HUS-related serogroups were present albeit at low frequencies. The non-O157 isolates were more heterogeneous than O157:H7 in their VG patterns, with 25/64 presenting VGs from both STEC and enterotoxigenic pathotypes (hybrid isolates). Of the STEC isolates, 62·5% were resistant at least to one antimicrobial, and no differences in AMR between O157:H7 and non-O157 were detected. All isolates had different profiles by PFGE and did not form a cluster. Overall, our results demonstrated that STEC in the cattle reservoir is still a matter of concern for human health due to the presence of HUS-related serogroups, the occurrence of certain VGs, AMR and the additional risks that hybrid isolates may pose, and thus warrants further investigation




Cabal A., Porrero MC., de la Cruz ML., Saez JL., Barcena C., Lopez G., Gortazar C., Dominguez L. y Alvarez J..




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Molecular characterization and antimicrobial resistance of STEC strains isolated from healthy cattle in 2011 and 2013 in Spain

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Molecular characterization and antimicrobial resistance of STEC strains isolated from healthy cattle in 2011 and 2013 in Spain


Participantes:

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Gobierno de Castilla-La ManchaSanidad y Biotecnología (SaBio). Instituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).

Ministerio de Agricultura, Alimentación y Medio AmbienteSubdirección General de Sanidad e Higiene Animal y Trazabilidad. Dirección General de Sanidad de la Producción Agraria. Ministerio de Agricultura, Alimentación y Medio Ambiente (MAGRAMA).

University of MinnesotaDepartment of Veterinary Population Medicine. College of Veterinary Medicine. University of Minnesota (UMM).







Epidemiology and Infection
FACTOR YEAR Q
2.075 2016

NLMID: 8703737

PMID: 27387818

ISSN: 0950-2688



TÍTULO: Molecular characterization and antimicrobial resistance of STEC strains isolated from healthy cattle in 2011 and 2013 in Spain


REVISTA: Epidemiol. Infect.


NUMERACIÓN: 144(14):2956-2966


AÑO: 2016


EDITORIAL: Cambridge University Press


AUTORES: Cabal A., Porrero MC., de la Cruz ML., Saez JL., Barcena C., Lopez G., Gortazar C., Dominguez L. and Alvarez J..


PARTICIPANTES VISAVET


5th
Carmen Bárcena Asensio
8th
Lucas Domínguez Rodríguez
Last
Julio Álvarez Sánchez

DOI: https://doi.org/10.1017/S0950268816001370


CITA ESTA PUBLICACIÓN:

Cabal A., Porrero MC., de la Cruz ML., Saez JL., Barcena C., Lopez G., Gortazar C., Dominguez L. y Alvarez J. Molecular characterization and antimicrobial resistance of STEC strains isolated from healthy cattle in 2011 and 2013 in Spain. Epidemiology and Infection. 144(14):2956-2966. 2016. (A). ISSN: 0950-2688. DOI: 10.1017/S0950268816001370


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