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Oral administration of heat-inactivated Mycobacterium bovis reduces the response of farmed red deer to avian and bovine tuberculin

Veterinary Immunology and Immunopathology publica este artículo de investigación

1 de abril de 2016

Orally delivered mycobacterial antigens may not sensitize the immunized animals causing a positive tuberculin skin test response. As the first step to address this critical issue, we characterized the response of farmed red deer (Cervus elaphus) to orally delivered heat-inactivated Mycobacterium bovis. Thirty-two adult red deer hinds from a farm known to be free of tuberculosis (TB) were randomly assigned to two different treatment groups, immunized (n = 24) and control (n = 8). Immunized hinds were dosed orally with 2 ml of PBS containing 6 × 106 heat-inactivated M. bovis. The mean skin test response of immunized deer to both avian purified protein derivative (aPPD) and bovine PPD (bPPD) was consistently lower in immunized than in control hinds. One year after immunization, immunized hinds had a significant reduction in the skin test response to aPPD and in the ELISA antibody levels against both aPPD and bPPD (24–36% reduction; P < 0.05). By contrast, no significant change was observed in the skin test response to phytohaemagglutinin, or in the ELISA antibody levels against the M. bovis specific antigen MPB70. The mRNA levels for C3, IFN-γ and IL-1β and serum protein levels for IFN-γ and IL-1β did not vary between immunized and control deer. However, serum C3 protein levels were significantly higher (P = 0.001) in immunized than in control deer six months after immunization. These results confirm that oral heat-inactivated M. bovis does not sensitize farmed red deer and therefore does not cause false-positive responses in the tuberculin skin test. The absence of sensitization in orally immunized deer opens the possibility of testing the vaccine in deer and possibly other ruminants without the risk of causing false-positive reactions in TB-tests. This study also provided the first evidence that orally-delivered inactivated mycobacterial antigens elicit some kind of immune response in a ruminant




Lopez V., Gonzalez-Barrio D., Lima-Barbero JF., Ortiz JA., Dominguez L., Juste R., Garrido JM., Sevilla I., Alberdi P., de la Fuente J. y Gortazar C.




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Oral administration of heat-inactivated Mycobacterium bovis reduces the response of farmed red deer to avian and bovine tuberculin

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Oral administration of heat-inactivated Mycobacterium bovis reduces the response of farmed red deer to avian and bovine tuberculin


Participantes:

Medianilla S.L.Medianilla S.L..

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Gobierno VascoNeiker-Tecnalia, Instituto Vasco de Investigación y Desarrollo Agrario. Gobierno Vasco.

Oklahoma State UniversityDepartament of Veterinary Pathobiology. Center for Veterinary Health Sciences (CVHS). Oklahoma State University (OSU).

Gobierno de Castilla-La ManchaSanidad y Biotecnología (SaBio). Instituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).

SabiotecSabiotec.







Veterinary Immunology and Immunopathology
FACTOR YEAR Q
1.718 2016

NLMID: 8002006

PMID: 27032499

ISSN: 0165-2427



TÍTULO: Oral administration of heat-inactivated Mycobacterium bovis reduces the response of farmed red deer to avian and bovine tuberculin


REVISTA: Vet. Immunol. Immunopathol.


NUMERACIÓN: 172(2016):21-25


AÑO: 2016


EDITORIAL: Elsevier Scientific


AUTORES: Lopez V., Gonzalez-Barrio D., Lima-Barbero JF., Ortiz JA., Dominguez L., Juste R., Garrido JM., Sevilla I., Alberdi P., de la Fuente J. and Gortazar C.


PARTICIPANTES VISAVET


Lucas Domínguez Rodríguez

DOI: https://doi.org/10.1016/j.vetimm.2016.03.003


CITA ESTA PUBLICACIÓN:

Lopez V., Gonzalez-Barrio D., Lima-Barbero JF., Ortiz JA., Dominguez L., Juste R., Garrido JM., Sevilla I., Alberdi P., de la Fuente J. y Gortazar C. Oral administration of heat-inactivated Mycobacterium bovis reduces the response of farmed red deer to avian and bovine tuberculin. Veterinary Immunology and Immunopathology. 172(2016):21-25. 2016. (A). ISSN: 0165-2427. DOI: 10.1016/j.vetimm.2016.03.003


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