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P22 protein complex in the serodiagnosis of animal tuberculosis: Antigenic stability and cross-reactivity with Corynebacterium pseudotuberculosis infection

Comparative Immunology, Microbiology and Infectious Diseases publica este artículo de investigación

1 de noviembre de 2022

The P22 ELISA was recently developed for the serodiagnosis of animal tuberculosis. Herein, the stability of the P22 antigen in different presentations and storage conditions, and the cross-reactivity with Corynebacterium pseudotuberculosis infection in small ruminants were evaluated. For the stability assay, serum samples from cows, sheep, goats, alpacas, badgers, and wild boar were used in the P22 ELISA. The cross-reactivity analysis used sera from sheep and goats with caseous lymphadenitis (CLA). Differences in the immune recognition of P22 were found when the antigen was stored at 40 °C, but without altering the negative or positive status of each sample. P22 ELISA presented 5.71 % cross-reactivity when CLA-positive sheep were evaluated, but no cross-reaction was observed among CLA-positive goat serum samples. This study showed that the P22 protein complex is stable under different formulations and temperatures, and that the assay presents a low cross-reactivity with CLA.
Keywords: Caseous lymphadenitis; Immunodiagnosis; Livestock; Mycobacterium tuberculosis complex; Wildlife; Zoonosis




Doria-Barral T., Infantes-Lorenzo JA., Moreno I., de Garnica-Garcia MG., Perez del Val B., Gortazar C., Meyer B., Portela RD., Dominguez L., Dominguez M. y Balseiro A.




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P22 protein complex in the serodiagnosis of animal tuberculosis: Antigenic stability and cross-reactivity with Corynebacterium pseudotuberculosis infection

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P22 protein complex in the serodiagnosis of animal tuberculosis: Antigenic stability and cross-reactivity with Corynebacterium pseudotuberculosis infection



Participantes:

Laboratório de Imunologia e Biologia Molecular. Instituto de Ciências da Saúde. Universidade Federal do Recôncavo da Bahia.

Instituto de Salud Carlos IIIServicio de Inmunología. Centro Nacional de Microbiología (CNM). Instituto de Salud Carlos III (ISCIII).

Universidad de LeónDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad de León.

Generalitat de CatalunyaCentre de Recerca en Sanitat Animal (CReSA). Universitat Autònoma de Barcelona (UAB). Instituto de Investigación y Tecnología Agroalimentarias (IRTA). Generalitat de Catalunya.

Gobierno de Castilla-La ManchaSanidad y Biotecnología (SaBio). Instituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Consejo Superior de Investigaciones CientíficasDepartamento de Sanidad Animal. Instituto de Ganadería de Montaña. Consejo Superior de Investigaciones Científicas (CSIC).







FACTOR YEAR Q
2.268 2021

NLMID: 7808924

PMID: 36265248

ISSN: 0147-9571



TÍTULO: P22 protein complex in the serodiagnosis of animal tuberculosis: Antigenic stability and cross-reactivity with Corynebacterium pseudotuberculosis infection


REVISTA: Comp Immunol Microbiol Infect Dis


NUMERACIÓN: 90-91:101891


AÑO: 2022


EDITORIAL: Elsevier Science


AUTORES: Doria-Barral T., Infantes-Lorenzo JA., Moreno I., de Garnica-Garcia MG., Perez del Val B., Gortazar C., Meyer B., Portela RD., Dominguez L., Dominguez M. and Balseiro A.


PARTICIPANTES VISAVET


Lucas Domínguez Rodríguez

DOI: https://doi.org/10.1016/j.cimid.2022.101891


CITA ESTA PUBLICACIÓN:

Doria-Barral T., Infantes-Lorenzo JA., Moreno I., de Garnica-Garcia MG., Perez del Val B., Gortazar C., Meyer B., Portela RD., Dominguez L., Dominguez M. y Balseiro A. P22 protein complex in the serodiagnosis of animal tuberculosis: Antigenic stability and cross-reactivity with Corynebacterium pseudotuberculosis infection. Comparative Immunology, Microbiology and Infectious Diseases. 90-91:101891. 2022. (A). ISSN: 0147-9571. DOI: 10.1016/j.cimid.2022.101891


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