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Pasteurella multocida isolates associated with ovine pneumonia are toxigenic

Veterinary Microbiology publica este artículo de investigación

1 de abril de 2019

The P. multocida toxin (PMT), a dermonecrotic protein encoded by the toxA gene, is the major virulence factor of capsular type D P. multocida strains causing progressive atrophic rhinitis (PAR) in pigs. A high frequency of P. multocida isolates harboring the toxA gene has been found among ovine pneumonic isolates, although the ability of these isolates to express PMT has never been examined. In this study we have investigated the ability of ovine toxA+ P. multocida isolates (n = 57) to express a functional toxin by detection of PMT toxin antigen using an ELISA test and its cytopathic effect in a Vero cell assay. PMT antigen was expressed in the great majority (54/57; 94.7%) of toxA+ isolates. Moreover, the 100% toxA+ ovine isolates analyzed produced a cytopathic effect in Vero cells within 24-48 h post-inoculation, identical to that described for porcine toxigenic P. multocida isolates. These results show for the first time that, in addition to isolates associated with PAR, isolates of P. multocida associated with pneumonia in sheep are also toxigenic. In addition, we found a total agreement (Kappa = 1; C.I. 0.75-1.25) between the detection of the toxA gene and the toxigenic capability of P. multocida isolates, indicating the PCR detection of toxA would be a suitable predictive marker of the toxigenic fitness of P. multocida




Cid D., Garcia-Alvarez A., Dominguez L., Fernandez-Garayzabal JF. y Vela AI..




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Pasteurella multocida isolates associated with ovine pneumonia are toxigenic

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Pasteurella multocida isolates associated with ovine pneumonia are toxigenic

Participantes:

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).







Veterinary Microbiology
FACTOR YEAR Q
2.791 2018

NLMID: 7705469

PMID: 31030847

ISSN: 0378-1135



TÍTULO: Pasteurella multocida isolates associated with ovine pneumonia are toxigenic


REVISTA: Vet. Microbiol.


NUMERACIÓN: 232:40_73


AÑO: 2019


EDITORIAL: Elsevier Science Publishers


AUTORES: Cid D., Garcia-Alvarez A., Dominguez L., Fernandez-Garayzabal JF. and Vela AI..


PARTICIPANTES VISAVET


3rd
Lucas Domínguez Rodríguez
4th
José Francisco Fernández-Garayzabal Fernández
Last
Ana Isabel Vela Alonso

DOI: https://doi.org/10.1016/j.vetmic.2019.04.006


CITA ESTA PUBLICACIÓN:

Cid D., Garcia-Alvarez A., Dominguez L., Fernandez-Garayzabal JF. y Vela AI. Pasteurella multocida isolates associated with ovine pneumonia are toxigenic. Veterinary Microbiology. 232:40_73. 2019. (A). ISSN: 0378-1135. DOI: 10.1016/j.vetmic.2019.04.006


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