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Phylogenetic analysis and geographical distribution of Theileria equi and Babesia caballi sequences from horses residing in Spain

Ticks and Tick-Borne Diseases publica este artículo de investigación

1 de noviembre de 2020

The intraerythrocytic protozoans Theileria equi and Babesia caballi are the causative agents of equine piroplasmosis (EP), one of the most important equine tick-borne diseases due to its significant impact on global international horse trade. Although EP is known to be endemic in Spain, previous phylogenetic studies have only been conducted for limited geographical regions. Therefore, the objective of this study was to evaluate the genetic diversity and distribution of these parasite species nationwide. This was performed by amplification of the 18S small subunit (SSU) rRNA gene from 100 EP positive equine blood samples using a nested PCR protocol, and sequencing the obtained amplicons. Seventy-seven T. equi and six B. caballi isolates were successfully sequenced and phylogenetic analysis revealed that the T. equi isolates grouped into the previously described clades A (n = 21/77), D (n = 1/77) and E (n = 55/77), while B. caballi isolates were placed into clades A (n = 5/6) and B (n = 1/6). Isolates from T. equi clade D and B. caballi clade B have not previously been reported in Spain. A greater intra-clade diversity (97.3–98.3 % identity) was observed between T. equi clade E isolates compared to those within clade A (99.7–100 % identity). Additionally, a multivariable logistic regression model was used to analyse associations between the clade of T. equi infection and available epidemiological data. Horses residing in Spanish northern regions were statistically more likely to be infected with T. equi clade E (p = 0.01). We conclude that while extensive sequence variation of equine piroplasms exists in Spanish infected horses, a requirement for increased equine movement controls between Spain and EP-endemic countries should be considered




Camino E., Cruz F., de Juan L., Dominguez L., Shiels B. y Coultous RM.




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Phylogenetic analysis and geographical distribution of Theileria equi and Babesia caballi sequences from horses residing in Spain




Participantes:

Universidad ComplutenseServicio de Vigilancia Sanitaria Equina (SEVISEQ). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

College of Medical. Institute of Biodiversity Animal Health and Comparative Medicine. Veterinary and Life Sciences. University of Glasgow.







Ticks and Tick-Borne Diseases
FACTOR YEAR Q
2.749 2019

ISSN: 1877-959X



TÍTULO: Phylogenetic analysis and geographical distribution of Theileria equi and Babesia caballi sequences from horses residing in Spain


REVISTA: Ticks and Tick-Borne Diseases


NUMERACIÓN: 11(6):101521


AÑO: 2020


EDITORIAL: ELSEVIER GMBH


AUTORES: Camino E., Cruz F., de Juan L., Dominguez L., Shiels B. and Coultous RM.


PARTICIPANTES VISAVET


First
Eliazar Camino Gutiérrez
2nd
Fátima Cruz López
3rd
Lucía de Juan Ferré
4th
Lucas Domínguez Rodríguez

DOI: https://doi.org/10.1016/j.ttbdis.2020.101521


CITA ESTA PUBLICACIÓN:

Camino E., Cruz F., de Juan L., Dominguez L., Shiels B. y Coultous RM. Phylogenetic analysis and geographical distribution of Theileria equi and Babesia caballi sequences from horses residing in Spain. Ticks and Tick-Borne Diseases. 11(6):101521. 2020. (A). ISSN: 1877-959X. DOI: 10.1016/j.ttbdis.2020.101521


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