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Use of liver transudation as an alternative matrix for the diagnosis of Hepatitis E using a commercial ELISA kit


4 de junio de 2018

Navarro A., de la Cruz ML., Barcena C., Hernandez M., Martinez I., Gonzalez S., Goyache J., Rodriguez-Lazaro D. y Garcia N.

Poster presentado en HEV2018 Hepatitis E: Paradigm of a food-borne zoonotic emerging disease in Europe



Hepatitis E is a viral disease currently considered an emerging zoonosis in industrialized countries. It is worldwide distributed and responsible of epidemics in developing countries and of sporadic outbreaks in developed countries. The transmission routes differ according to the area of appearance. Thus, in developing countries the transmission results from the consumption of contaminated water, while in developed countries is linked to the consumption of raw or undercooked food, specially pig liver and derived products, but also wild boar and deer ones. The most commonly used diagnostic technique is the ELISA test in serum samples, although ELISAs in meat juice samples have also been described. However, in certain cases the optimal collection of serum is not possible.
The aim of the present study is to validate the use of the liver transudate as an alternative matrix to serum for diagnosis of Hepatitis E in animals, as well to determinate the optimal dilution to be used.
For its implementation, sera and liver transudate samples were collected from 94 animals: 25 iberian pigs, 25 wild boars and 44 white pigs. A commercial ELISA kit (iDvet) was used for the analysis of both type of samples. Results obtained from the serum analysis were compared with those of undiluted transudate as well as at different dilutions (1:2, 1:10, 1:20). The agreement between the qualitative results (sera vs. liver transudate at different dilutions) was measured using the Kappa statistic and, for the comparison of the corresponding optical densities, the Intraclass Correlation Coefficient (ICC) was employed.
The highest agreement, according to the ELISA results in sera samples, was observed with liver transudate at a dilution of 1:10, in terms of both seropositivity and optical densities, with kappa coefficients and ICC higher than 0.728 and 0.864, respectively, even though slightly differences were observed between iberian pig and wild boar with the white pigs.
Our results demonstrate that liver transudate at a 1:10 dilution may be an interesting alternative for the detection of anti-Hepatitis E virus antibodies by ELISA in swine and wild boars, although further studies should be carried out in this regar



Participantes:

Universidad ComplutenseServicio de Zoonosis Emergentes, de Baja Prevalencia y Agresivos Biológicos (NED). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad de BurgosUnidad de Microbiología. Departamento de Biotecnología y Ciencia de los Alimentos. Universidad de Burgos (UBU).

Instituto Tecnológico Agrario de Castilla y LeónLaboratorio de Biología Molecular y Microbiología. Instituto Tecnológico Agrario de Castilla y León (ITACyL).


Enlace a HEV2018 Hepatitis E: Paradigm of a food-borne zoonotic emerging disease in Europe





publiDB

HEV2018 Hepatitis E: Paradigm of a food-borne zoonotic emerging disease in Europe


4-5 junio de 2018
Madrid
España

TÍTULO: Use of liver transudation as an alternative matrix for the diagnosis of Hepatitis E using a commercial ELISA kit


TIPO: Comunicación en póster


AUTORES: Navarro A., de la Cruz ML., Barcena C., Hernandez M., Martinez I., Gonzalez S., Goyache J., Rodriguez-Lazaro D. y Garcia N.


PARTICIPANTES VISAVET


First
Alejandro Navarro Gómez
2nd
Mª Luisa de la Cruz Conty
3rd
Carmen Bárcena Asensio
5th
Irene Martínez Alares
6th
Sergio González Domínguez
7th
Joaquín Goyache Goñi
Last
Nerea García Benzaquén

FECHA: 4 de junio de 2018



CITA ESTA COMUNICACIÓN:

Navarro A., de la Cruz ML., Barcena C., Hernandez M., Martinez I., Gonzalez S., Goyache J., Rodriguez-Lazaro D. y Garcia N. Use of liver transudation as an alternative matrix for the diagnosis of Hepatitis E using a commercial ELISA kit. HEV2018 Hepatitis E: Paradigm of a food-borne zoonotic emerging disease in Europe, VISAVET Centro de Vigilancia Sanitaria Veterinaria, Servicio de Zoonosis Emergentes, de Baja Prevalencia y Agresivos Biológicos. Centro de Vigilancia Sanitaria Veterinaria. Universidad Complutense, Med-Vet-Net Association, Madrid, España, 4 de junio de 2018. (Comunicación en póster)


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