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A pathological study of Leishmania infantum natural infection in European rabbits (Oryctolagus cuniculus) and Iberian hares (Lepus granatensis)

Investigation article published in Transboundary and Emerging Diseases

July 24th, 2019

In this study, we describe the pathology of Leishmania infantum infection in naturally infected wild Leporidae and compare diagnosis of infection using histopathology, direct fluorescent antibody (DFA) assay, immunofluorescence antibody test (IFAT), and quantitative real‐time PCR (qPCR). Tissues were analysed from 52 European rabbits (Oryctolagus cuniculus) and 7 Iberian hares (Lepus granatensis) from the Community of Madrid (Spain). Our results show that L. infantum infection is associated with only minimal histopathological lesions and that L. infantum amastigotes can be detected by DFA assay in all tissues types tested, including skin. These results were confirmed by qPCR on fresh frozen tissues in 13% of rabbits and 100% of hares. However, L. infantum DNA could not be detected by qPCR on paraffin‐embedded tissue obtained by laser capture microdissection. Using the DFA assay to diagnose L. infantum infection may provide further insights into this disease in wild animals and may allow the precise tissue localization of L. infantum, thereby guiding follow‐up tests with more accurate qPCR




Ortega-García MV., Salguero FJ., Rodriguez-Bertos A., Moreno I., Garcia N., Garcia-Seco T., Torre G., Dominguez L. and Dominguez M.




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A pathological study of Leishmania infantum natural infection in European rabbits (Oryctolagus cuniculus) and Iberian hares (Lepus granatensis)

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A pathological study of Leishmania infantum natural infection in European rabbits (Oryctolagus cuniculus) and Iberian hares (Lepus granatensis)


Participants:

Ministerio de DefensaSubdirección de Sistemas Terrestres. Instituto Nacional de Técnica Aeroespacial (INTA). Ministerio de Defensa.

University of SurreySchool of Veterinary Medicine. Faculty of Health and Medical Sciences. University of Surrey.

Public Health EnglandPublic Health England (PHE).

Universidad ComplutenseDepartamento de Medicina y Cirugía Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Instituto de Salud Carlos IIIServicio de Inmunología. Centro Nacional de Microbiología (CNM). Instituto de Salud Carlos III (ISCIII).

Universidad ComplutenseServicio de Patología y Veterinaria Forense (SAP). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseServicio de Zoonosis Emergentes, de Baja Prevalencia y Agresivos Biológicos (NED). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).







Transboundary and Emerging Diseases
FACTOR YEAR Q
3.554 2018

NLMID: 101319538

PMID: 31339665

ISSN: 1865-1674



TITLE: A pathological study of Leishmania infantum natural infection in European rabbits (Oryctolagus cuniculus) and Iberian hares (Lepus granatensis)


JOURNAL: Transbound. Emerg. Dis.


NUMERACIÓN: 1-8


AÑO: 2019


PUBLISHER: Blackwell Verlag


AUTHORS: Ortega-García MV., Salguero FJ., Rodriguez-Bertos A., Moreno I., Garcia N., Garcia-Seco T., Torre G., Dominguez L. and Dominguez M.


VISAVET PARTICIPANTS


First
Mª Victoria Ortega García
3rd
Antonio Manuel Rodríguez Bertos
5th
Nerea García Benzaquén
6th
Teresa García-Seco Romero
7th
Gabriela Luz Torre Cama
8th
Lucas Domínguez Rodríguez

DOI: https://doi.org/ 10.1111/tbed.13305


CITE THIS PUBLICATION:

Ortega-García MV., Salguero FJ., Rodriguez-Bertos A., Moreno I., Garcia N., Garcia-Seco T., Torre G., Dominguez L. and Dominguez M. A pathological study of Leishmania infantum natural infection in European rabbits (Oryctolagus cuniculus) and Iberian hares (Lepus granatensis). Transboundary and Emerging Diseases. 1-8. 2019. (A). ISSN: 1865-1674. DOI: 10.1111/tbed.13305


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