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Detailed chronological analysis of microevolution events in herds infected persistently by Mycobacterium bovis

Investigación publicada en Veterinary Microbiology

1 de febrero de 2016

Various studies have analyzed microevolution events leading to the emergence of clonal variants in human infections by Mycobacterium tuberculosis. However, microevolution events in animal tuberculosis remain unknown. We performed a systematic analysis of microevolution events in eight herds that were chronically infected by Mycobacterium bovis for more than 12 months. We analyzed 88 animals using a systematic screening procedure based on discriminatory MIRU-VNTR genotyping at sequential time points during the infection. Microevolution was detected in half of the herds studied. Emergence of clonal variants did not require long infection periods or a high number of infected animals in the herd. Microevolution was not restricted to strains from specific spoligotypes, and the subtle variations detected involved different MIRU loci. The genetic locations of the subtle genotypic variations recorded in the clonal variants indicated potential functional significance. This finding was consistent with the dynamics of some clonal variants, which outcompeted the original strains, suggesting an advantageous phenotype. Our data constitute a first step in defining the thresholds of variability to be tolerated in molecular epidemiology studies of M. bovis. We could therefore ensure that related clonal variants emerging as a result of microevolution events are not going to be misinterpreted as unrelated isolates




Navarro Y., Romero B., Bouza E., Dominguez L., de Juan L. y Garcia de Viedma D.




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Detailed chronological analysis of microevolution events in herds infected persistently by Mycobacterium bovis

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Detailed chronological analysis of microevolution events in herds infected persistently by Mycobacterium bovis


Participantes:

Comunidad de MadridServicio de Microbiología y Enfermedades Infecciosas. Instituto de Investigación Sanitaria Gregorio Marañón (IISGM). Hospital General Universitario Gregorio Marañón. Consejería de Sanidad. Comunidad de Madrid.

Instituto de Salud Carlos IIICentro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES). Instituto de Salud Carlos III (ISCIII).

CEI Campus MoncloaCEI Campus Moncloa.

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseFacultad de Medicina. Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







Veterinary Microbiology
FACTOR YEAR Q
2.628 2016

NLMID: 7705469

PMID: 26790941

ISSN: 0378-1135



TÍTULO: Detailed chronological analysis of microevolution events in herds infected persistently by Mycobacterium bovis


REVISTA: Vet. Microbiol.


NUMERACIÓN: 183(2016):97-102


AÑO: 2016


EDITORIAL: Elsevier Science Publishers


AUTORES: Navarro Y., Romero B., Bouza E., Dominguez L., de Juan L. and Garcia de Viedma D.


PARTICIPANTES VISAVET


2nd
Beatriz Romero Martínez
4th
Lucas Domínguez Rodríguez
5th
Lucía de Juan Ferré

DOI: https://doi.org/10.1016/j.vetmic.2015.11.032


CITA ESTA PUBLICACIÓN:

Navarro Y., Romero B., Bouza E., Dominguez L., de Juan L. y Garcia de Viedma D. Detailed chronological analysis of microevolution events in herds infected persistently by Mycobacterium bovis. Veterinary Microbiology. 183(2016):97-102. 2016. (A). ISSN: 0378-1135. DOI: 10.1016/j.vetmic.2015.11.032


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