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Direct PCR in Bovine Tissue Samples to Detect the Mycobacterium tuberculosis Complex: time for change

Comunicación oral en Seventh International Conference on Mycobacterium bovis

8 de junio de 2022

Lorente-Leal V., Gutierrez A., Liandris E., Dominguez L., Bezos J., de Juan L. y Romero B.

Objective. Real-Time PCR is considered a promising technique in the detection of Mycobacterium tuberculosis complex (MTBC) species in bovine tissue samples as it is a sensitive and specific method that yields results in a reduced period of time and is not subjected to the limitations of microbiological culture. The objective of this study was to evaluate the diagnostic performance of two Real-Time PCRs based on the IS6110 and mpb70 molecular targets in the detection and quantification of MTBC DNA.
Key results/findings. Nine-hundred and eighty-five (IS6110) and 200 (mpb70) bovine tissue samples were processed and analysed in parallel through microbiological culture and Real-Time PCR. When compared to culture, diagnostic sensitivity was 96.78% (95% CI: 94.46% - 98.15%) for the IS6110 PCR and 89.19% (95% CI: 79.80% to 95.22%) for the mpb70 PCR, whereas diagnostic specificity was 99.02% (95% CI: 97.88% - 99.55%) and 96.03% (95% CI: 90.98% to 98.70%), respectively. Minor cross-reactivity was detected for the IS6110 PCR against a reduced number of Mycobacterium avium subsp. hominissuis isolates. Diagnostic performance of the IS6110 PCR was moderate and very high in samples with non-visible and visible lesions, respectively.
Significance/Impact of study. The Real-Time PCRs presented here show high diagnostic performance when compared to culture, indicating that they are suitable as first-line techniques in the detection of MTBC species in bovine tissue samples. The single-copy nature of the mpb70 makes it also an appealing quantification technique with multiple applications. The high performance of the IS6110 PCR in samples with visible lesions makes this method an alternative to culture in this type of samples





Participantes:

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).


Enlace a Seventh International Conference on Mycobacterium bovis





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Seventh International Conference on Mycobacterium bovis


Seventh International Conference on Mycobacterium bovis
7-10 junio de 2022
Galway
Irlanda

TÍTULO: Direct PCR in Bovine Tissue Samples to Detect the Mycobacterium tuberculosis Complex: time for change


TIPO: Comunicación oral


AUTORES: Lorente-Leal V., Gutierrez A., Liandris E., Dominguez L., Bezos J., de Juan L. y Romero B.


PARTICIPANTES VISAVET


First
Victor Lorente Leal
2nd
Alexandra Gutiérrez Tobaruela
4th
Lucas Domínguez Rodríguez
5th
Javier Bezos Garrido
6th
Lucía de Juan Ferré
Last
Beatriz Romero Martínez

FECHA: 8 de junio de 2022



CITA ESTA COMUNICACIÓN:

Lorente-Leal V., Gutierrez A., Liandris E., Dominguez L., Bezos J., de Juan L. y Romero B. Direct PCR in Bovine Tissue Samples to Detect the Mycobacterium tuberculosis Complex: time for change. Seventh International Conference on Mycobacterium bovis, Meet in Ireland, Failte Ireland, Galway, Irlanda, 8 de junio de 2022. (Comunicación oral)


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