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Effect of heat-inactivated Mycobacterium avium subspecies paratuberculosis (MAP) vaccine on the lesions and immunopathology developed in target tissues of naturally MAP-infected goats

Investigación publicada en Veterinary Microbiology

1 de octubre de 2022

Paratuberculosis (PTB) is a disease caused by Mycobacterium avium subspecies paratuberculosis (MAP), which affects a broad range of hosts, including domestic and wild animals. PTB is a chronic granulomatous enteritis and lymphadenitis that compromises animal welfare and causes economic losses. The objective of this study was to evaluate the effect of a commercial heat-inactivated MAP vaccine on lesions and immunopathology developed in the target tissues of goats naturally infected with MAP. Lesions compatible with PTB in the intestine and regional lymph nodes (LNs), as well as local immune response to MAP, were evaluated and compared in Gudair®-vaccinated (n = 14) and unvaccinated (n = 11) goats from a MAP-infected farm. The percentage of animals with multifocal granulomatous lesions in the jejunal (p = 0.05) and ileocecal (p = 0.02) LNs was higher in unvaccinated animals, while a lesion score reduction of 50% was found in the LNs of vaccinated animals. Unvaccinated animals showed increased numbers and wider distribution of macrophages (MΦs, CD68 +) in histiocytic infiltrate (p = 0.0003), associated with increased numbers of mycobacteria. Increased inducible nitric oxide synthase (iNOS) expression was also reported in these animals, while M2 MΦs (CD163 +) were scarce in both groups. Vaccinated animals showed an increase in CD3 + lymphocytes, although differences in interferon gamma (IFNγ) were negligible. These results support the hypothesis that heat-inactivated MAP vaccination could reduce the severity of PTB lesions and mycobacterial load in target tissues in vaccinated adult goats




Agullo-Ros I., Andrada M., Perez-Sancho M., Roy A., Bezos J., Bonnet T., Moreno I., Paz-Sanchez Y., Dominguez M., Gomez-Villamandos JC., Dominguez L. y Risalde MA.




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Effect of heat-inactivated Mycobacterium avium subspecies paratuberculosis (MAP) vaccine on the lesions and immunopathology developed in target tissues of naturally MAP-infected goats

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Effect of heat-inactivated Mycobacterium avium subspecies paratuberculosis (MAP) vaccine on the lesions and immunopathology developed in target tissues of naturally MAP-infected goats



Participantes:

Universidad de CórdobaDepartamento de Anatomía y Anatomía Patológica Comparadas. Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad de Córdoba (UCO).

Universidad de Las Palmas de Gran CanariaInstituto Universitario de Sanidad Animal y Seguridad Alimentaria (IUSA). Facultad de Veterinaria. Universidad de Las Palmas de Gran Canaria (ULPGC).

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Instituto de Salud Carlos IIIServicio de Inmunología. Centro Nacional de Microbiología (CNM). Instituto de Salud Carlos III (ISCIII).

Instituto de Salud Carlos IIICentro de Investigación de Enfermedades Infecciosas (CIBERINFEC). Instituto de Salud Carlos III (ISCIII).







Veterinary Microbiology
FACTOR YEAR Q
3.246 2021

NLMID: 7705469

PMID: 36037619

ISSN: 0378-1135



TÍTULO: Effect of heat-inactivated Mycobacterium avium subspecies paratuberculosis (MAP) vaccine on the lesions and immunopathology developed in target tissues of naturally MAP-infected goats


REVISTA: Vet. Microbiol.


NUMERACIÓN: 273:109543


AÑO: 2022


EDITORIAL: Elsevier Science Publishers


AUTORES: Agullo-Ros I., Andrada M., Perez-Sancho M., Roy A., Bezos J., Bonnet T., Moreno I., Paz-Sanchez Y., Dominguez M., Gomez-Villamandos JC., Dominguez L. and Risalde MA.


PARTICIPANTES VISAVET


3rd
Marta Pérez Sancho
5th
Javier Bezos Garrido
11th
Lucas Domínguez Rodríguez

DOI: https://doi.org/10.1016/j.vetmic.2022.109543


CITA ESTA PUBLICACIÓN:

Agullo-Ros I., Andrada M., Perez-Sancho M., Roy A., Bezos J., Bonnet T., Moreno I., Paz-Sanchez Y., Dominguez M., Gomez-Villamandos JC., Dominguez L. y Risalde MA. Effect of heat-inactivated Mycobacterium avium subspecies paratuberculosis (MAP) vaccine on the lesions and immunopathology developed in target tissues of naturally MAP-infected goats. Veterinary Microbiology. 273:109543. 2022. (A). ISSN: 0378-1135. DOI: 10.1016/j.vetmic.2022.109543


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