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Environment and Offspring Surveillance in Porcine Brucellosis

Investigación publicada en Frontiers in veterinary science

21 de junio de 2022

Porcine brucellosis, caused by Brucella suis (B. suis), is a notifiable disease causing significant economic losses in production systems. Most infected pigs may act as carriers and shed B. suis even if asymptomatic. This can contribute to environmental persistence, thus hindering control efforts. Here, the environment and the offspring were investigated during and after a B. suis outbreak at a sow breeding farm. The diagnosis of B. suis in sows (n = 1,140) was performed by culture and polymerase chain reaction (PCR) from vaginal swabs, indirect enzyme-linked immunosorbent assay (I-ELISA) from sera, and brucellin skin test (BST). B. suis diagnosis in post-weaning pigs (n = 899) was performed by I-ELISA in sera and BST. The environmental surveillance programme was implemented by placing gauze sponges (n = 175) pre-hydrated in a surfactant and inactivating liquid for Brucella DNA detection by PCR in different farm areas. Our results showed that the offspring of infected sows reacted to in vivo techniques for B. suis. Furthermore, the offspring born during the outbreak displayed higher seropositivity (I-ELISA) and reactivity (BST) than those pigs born after. Brucella DNA was detected in pregnant sow areas, boxes, boots, and post-weaning pig areas. In addition, Brucella DNA environmental detection was higher during the B. suis outbreak than the post B. suis outbreak. The environmental approach has proven to be a simple, practical, valuable, and safe method to detect and monitor B. suis. These results suggest a role of the environment and the offspring that should be considered in porcine brucellosis surveillance and control programmes




Rebollada A., Perez-Sancho M., Rodriguez-Bertos A., Garcia N., Martinez I., Navarro A., Dominguez L. y Garcia-Seco T..




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Environment and Offspring Surveillance in Porcine Brucellosis

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Environment and Offspring Surveillance in Porcine Brucellosis



Participantes:

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Medicina y Cirugía Animal. Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







Frontiers in veterinary science
FACTOR YEAR Q
3.471 2021

NLMID: 101666658

PMID: 35799841

ISSN: 2297-1769



TÍTULO: Environment and Offspring Surveillance in Porcine Brucellosis


REVISTA: Front Vet Sci


NUMERACIÓN: 9:915692


AÑO: 2022


EDITORIAL: Lausanne : Frontiers Media S.A


AUTORES: Rebollada A., Perez-Sancho M., Rodriguez-Bertos A., Garcia N., Martinez I., Navarro A., Dominguez L. and Garcia-Seco T..


PARTICIPANTES VISAVET


First
Agustín Miguel Rebollada Merino
2nd
Marta Pérez Sancho
3rd
Antonio Manuel Rodríguez Bertos
4th
Nerea García Benzaquén
5th
Irene Martínez Alares
7th
Lucas Domínguez Rodríguez
Last
Teresa García-Seco Romero

DOI: https://doi.org/10.3389/fvets.2022.915692


CITA ESTA PUBLICACIÓN:

Rebollada A., Perez-Sancho M., Rodriguez-Bertos A., Garcia N., Martinez I., Navarro A., Dominguez L. y Garcia-Seco T. Environment and Offspring Surveillance in Porcine Brucellosis. Frontiers in veterinary science. 9:915692. 2022. (A). ISSN: 2297-1769. DOI: 10.3389/fvets.2022.915692


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