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Detection of anti-Leishmania infantum antibodies in sylvatic lagomorphs from an epidemic area of Madrid using the indirect immunofluorescence antibody test

Investigación publicada en Veterinary Parasitology

31 de enero de 2014

An outbreak of human leishmaniosis was confirmed in the southwest of the province of Madrid, Spain, between July 2009 and December 2012. Incidence of Leishmania infection in dogs was unchanged in this period, prompting a search for alternative sylvatic infection reservoirs. We evaluated exposure to Leishmania in serum samples from animals in the area with an indirect immunofluorescence test (IFAT). Using promastigotes from six culture passages and a 1/25 threshold titer, we found anti-L. infantum seroreactivity in 9.3% of cats (4 of 43), 45.7% of rabbits (16/35) and 74.1% of hares (63/85). Use of promastigotes from >10 in vitro passages resulted in a notably IFAT lower titer, suggesting antigenic changes during extended culture. Postmortem inspection of seropositive animals showed no clinical signs of infection. The results clearly suggest that asymptomatic hares were the main reservoir in the outbreak, and corroborate IFAT as a sensitive serological surveillance method to detect such cryptic Leishmania infections




Moreno I., Alvarez J., Garcia N., de la Fuente S., Martinez I., Marino E., Torano A., Goyache J., Vilas F., Dominguez L. y Dominguez M.




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Detection of anti-Leishmania infantum antibodies in sylvatic lagomorphs from an epidemic area of Madrid using the indirect immunofluorescence antibody test

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Detection of anti-Leishmania infantum antibodies in sylvatic lagomorphs from an epidemic area of Madrid using the indirect immunofluorescence antibody test



Participantes:

Instituto de Salud Carlos IIIServicio de Inmunología. Centro Nacional de Microbiología (CNM). Instituto de Salud Carlos III (ISCIII).

Universidad ComplutenseServicio de Zoonosis Emergentes, de Baja Prevalencia y Agresivos Biológicos (NED). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Comunidad de MadridDirección General de Ordenación e Inspección. Consejería de Sanidad. Comunidad de Madrid.

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Comunidad de MadridInstituto Ramón y Cajal de Investigación Sanitaria (IRYCIS). Universidad Complutense (UCM). Universidad Autónoma de Madrid (UAM). Universidad de Alcalá (UAH). Salud Madrid. Comunidad de Madrid.







Veterinary Parasitology
FACTOR YEAR Q
2.460 2014

NLMID: 7602745

PMID: 24211046

ISSN: 0304-4017



TÍTULO: Detection of anti-Leishmania infantum antibodies in sylvatic lagomorphs from an epidemic area of Madrid using the indirect immunofluorescence antibody test


REVISTA: Vet Parasitol


NUMERACIÓN: 199(3-4):264-7


AÑO: 2014


EDITORIAL: Elsevier


AUTORES: Moreno I., Alvarez J., Garcia N., de la Fuente S., Martinez I., Marino E., Torano A., Goyache J., Vilas F., Dominguez L. and Dominguez M.


First
Inmaculada Moreno Iruela
2nd
Julio Álvarez Sánchez
3rd
Nerea García Benzaquén
5th
Irene Martínez Alares
8th
Joaquín Goyache Goñi
10th
Lucas Domínguez Rodríguez
Last
Mercedes Domínguez Rodríguez

DOI: https://doi.org/10.1016/j.vetpar.2013.10.010


CITA ESTA PUBLICACIÓN:

Moreno I., Alvarez J., Garcia N., de la Fuente S., Martinez I., Marino E., Torano A., Goyache J., Vilas F., Dominguez L. y Dominguez M. Detection of anti-Leishmania infantum antibodies in sylvatic lagomorphs from an epidemic area of Madrid using the indirect immunofluorescence antibody test. Veterinary Parasitology. 199(3-4):264-7. 2014. (A). ISSN: 0304-4017. DOI: 10.1016/j.vetpar.2013.10.010


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