Artículo de investigación publicado en Clinical and Vaccine Immunology

1 de agosto de 2014

Mycobacterium bovis causes animal tuberculosis (TB) in cattle, humans, and other mammalian species, including pigs. The goal of this study was to experimentally assess the response of pigs with and without tonsillectomy to oral vaccination with heat-inactivated M. bovis and challenge with a virulent M. bovis field strain; to compare pig and wild boar responses using the same vaccination model as previously used in Eurasian wild boar (Sus scrofa); and to evaluate the use of several ELISA and lateral flow tests for in vivo TB diagnosis in pigs, and verify if these tests are influenced by oral vaccination with inactivated M. bovis. At necropsy, lesion and culture scores were 20-43% higher in the controls compared to vaccinated pigs. Massive M. bovis growth from thoracic tissue samples was observed in 4 out of 9 controls, but in none of 10 vaccinated pigs. No effect of the tonsils was observed on these scores, suggesting that tonsils are not involved in the protective response to this vaccine in pigs. Serum antibody levels increased significantly only after challenge. At necropsy, the estimated sensitivity of the ELISAs and DPP assays ranged from 89% to 94%. In the oral mucosa, no differences in gene expression were observed in the control group between pigs with and without tonsils. In the vaccinated group, the mRNA levels for CCR7, IFN-β and MUT were higher in pigs with tonsils. Complement factor 3 mRNA levels in PBMC increased with vaccination and decreased after M. bovis challenge. This information is relevant for pig production in M. bovis endemic regions and for TB vaccine research

Beltran-Beck B., Romero B., Boadella M., Casal C., Bezos J., Mazariegos M., Martin M., Galindo RC., Perez de la Lastra JM., Villar M., Garrido JM., Sevilla AI., Asensio F., Sicilia J., Lyashchenko KP., Dominguez L., Juste RA., de la Fuente J. y Gortazar C.

Tonsils of the soft palate do not mediate the response of pigs to oral vaccination with heat inactivated Mycobacterium bovis

Tonsils of the soft palate do not mediate the response of pigs to oral vaccination with heat inactivated Mycobacterium bovis

	Sanidad y Biotecnología (SaBio). Instituto de Investigación en Recursos Cinegéticos (IREC). Universidad de Castilla La Mancha (UCLM). Consejo Superior de Investigaciones Científicas (CSIC). Gobierno de Castilla-La Mancha (JCCM).
	Servicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).
	Center for Veterinary Health Sciences (CVHS). Oklahoma State University (OSU).
	Instituto Vasco de Investigación y Desarrollo Agrario (NEIKER). Gobierno Vasco.
	Hospital General Universitario Gregorio Marañón. Consejería de Sanidad. Comunidad de Madrid.
	Chembio Diagnostic Systems Inc.