Inicio \ Divulgación \


Assessment of genetic diversity of zoonotic Brucella spp. recovered from livestock in Egypt using multiple locus VNTR analysis

Comunicación presentada en 6th Pan Commonwealth Veterinary Conference of the CVA and the 27th Congress of the Veterinary Association of Malaysia

23 de marzo de 2015

Menshawy AMS., Perez-Sancho M., Garcia-Seco T., Hosein HI., Garcia N., Martinez I., Sayour AE., Goyache J., Azzam RA., Dominguez L. y Alvarez J.

Brucellosis is endemic in most parts of Egypt, where it is caused mainly by Brucella melitensis biovar 3, and affects cattle and small ruminants in spite of ongoing efforts devoted to its control. Knowledge of the predominant Brucella species/strains circulating in a region is a prerequisite of a brucellosis control strategy. For this reason a study aiming at the evaluation of the phenotypic and genetic heterogeneity of a panel of 17 Brucella spp. isolates recovered from domestic ruminants (cattle, buffalo, sheep, and goat) from four governorates during a period of five years (2002-2007) was carried out using microbiological tests and molecular biology techniques (PCR, MLVA-15, and sequencing). Thirteen strains were identified as B. melitensis biovar 3 while all phenotypic and genetic techniques classified the remaining isolates as B. abortus (n = 2) and B. suis biovar 1 (n = 2). MLVA-15 yielded a high discriminatory power (h = 0.801), indicating a high genetic diversity among the B. melitensis strains circulating among domestic ruminants in Egypt. This is the first report of the isolation of B. suis from cattle in Egypt which, coupled with the finding of B. abortus, suggests a potential role of livestock as reservoirs of several zoonotic Brucella species in the region




Participantes:

Beni-Suef UniversityFaculty of Veterinary Medicine. Beni-Suef University.

Universidad ComplutenseServicio de Zoonosis Emergentes, de Baja Prevalencia y Agresivos Biológicos (NED). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Animal Health Research InstituteDepartment of Brucellosis. Animal Health Research Institute (AHRI).

Comunidad de MadridInstituto Ramón y Cajal de Investigación Sanitaria (IRYCIS). Universidad Complutense (UCM). Universidad Autónoma de Madrid (UAM). Universidad de Alcalá (UAH). Salud Madrid. Comunidad de Madrid.


Enlace a 6th Pan Commonwealth Veterinary Conference of the CVA and the 27th Congress of the Veterinary Association of Malaysia





publiDB

6th Pan Commonwealth Veterinary Conference of the CVA and the 27th Congress of the Veterinary Association of Malaysia


23-27 marzo de 2015
Kuala Lumpur
Malaysia

TÍTULO: Assessment of genetic diversity of zoonotic Brucella spp. recovered from livestock in Egypt using multiple locus VNTR analysis


TIPO: Comunicación en póster


AUTORES: Menshawy AMS., Perez-Sancho M., Garcia-Seco T., Hosein HI., Garcia N., Martinez I., Sayour AE., Goyache J., Azzam RA., Dominguez L. y Alvarez J.


PARTICIPANTES VISAVET


2nd
Marta Pérez Sancho
3rd
Teresa García-Seco Romero
5th
Nerea García Benzaquén
6th
Irene Martínez Alares
8th
Joaquín Goyache Goñi
10th
Lucas Domínguez Rodríguez
Last
Julio Álvarez Sánchez

FECHA: 23 de marzo de 2015



CITA ESTA COMUNICACIÓN:

Menshawy AMS., Perez-Sancho M., Garcia-Seco T., Hosein HI., Garcia N., Martinez I., Sayour AE., Goyache J., Azzam RA., Dominguez L. y Alvarez J. Assessment of genetic diversity of zoonotic Brucella spp. recovered from livestock in Egypt using multiple locus VNTR analysis. 6th Pan Commonwealth Veterinary Conference of the CVA and the 27th Congress of the Veterinary Association of Malaysia , Commonwealth Veterinary Association, Veterinary Association Malaysia, Kuala Lumpur, Malaysia, 23 de marzo de 2015. (Comunicación en póster)


SERVICIOS: