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Effect of a recent parenteral dexamethasone and ketoprofen administration on the immunological diagnosis of tuberculosis in goats

Investigación publicada en Frontiers in veterinary science

10 de noviembre de 2022

Caprine tuberculosis (TB) is a zoonosis caused by members of the Mycobacterium tuberculosis complex (MTBC). Caprine TB eradication programmes are based mainly on intradermal tuberculin tests and slaughterhouse surveillance. Different factors may affect the performance of the TB diagnostic tests used in caprine herds and, therefore, their ability to detect infected animals. The present study evaluates the effect of the fraudulent administration of two anti-inflammatory substances, dexamethasone and ketoprofen, on the performance of the TB diagnostic techniques used in goats, as well as the suitability of high performance liquid chromatography (HPLC) for their detection in hair samples. The animals (n = 90) were distributed in three groups: (1) a group treated with dexamethasone (n = 30); a second group treated with ketoprofen (n = 30); and a third non-treated control group (n = 30). Both dexamethasone and ketoprofen groups were subjected to intramuscular inoculation with the substances 48 h after the administration of bovine and avian purified protein derivatives (PPDs), that is, 24 h before the tests were interpreted. All the animals were subjected to the single and comparative intradermal tuberculin (SIT and CIT, respectively) tests, interferon-gamma release assay (IGRA) and P22 ELISA. The number of SIT test reactors was significantly lower in the dexamethasone (p = 0.001) and ketoprofen (p < 0.001) groups 72 h after the bovine PPD inoculation compared with the control group. A significantly higher number of positive reactors to IGRA was detected within the dexamethasone group (p = 0.016) 72 h after PPD administration compared to the control group. Dexamethasone and ketoprofen detection in either hair or serum samples was challenging when using HPLC since these substances were not detected in animals whose skin fold thickness (SFT) was reduced, what could be an issue if they are used for fraudulent purposes. In conclusion, the parenteral administration of dexamethasone or ketoprofen 48 h after the PPDs administration can significantly reduce the increase in SFT (mm) and subsequently the number of positive reactors to SIT test




Ortega J., de Juan L., Sevilla IA., Garrido JM., Velasco C., Romero B., Dominguez M., Perez del Val B., Nebot C., Saez-Llorente JL., Alvarez J. y Bezos J..




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Effect of a recent parenteral dexamethasone and ketoprofen administration on the immunological diagnosis of tuberculosis in goats





Participantes:

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Gobierno VascoInstituto Vasco de Investigación y Desarrollo Agrario (NEIKER). Gobierno Vasco.

Basque Research and Technology Alliance (BRTA).

Instituto de Salud Carlos IIIServicio de Inmunología. Centro Nacional de Microbiología (CNM). Instituto de Salud Carlos III (ISCIII).

Generalitat de CatalunyaCentre de Recerca en Sanitat Animal (CReSA). Universitat Autònoma de Barcelona (UAB). Instituto de Investigación y Tecnología Agroalimentarias (IRTA). Generalitat de Catalunya.

Universidad de Santiago de CompostelaFacultad de Veterinaria. Universidad de Santiago de Compostela (USC).

Ministerio de Agricultura, Pesca y AlimentaciónMinisterio de Agricultura, Pesca y Alimentación (MAPA).







Frontiers in veterinary science
FACTOR YEAR Q
3.471 2021

NLMID: 101666658

ISSN: 2297-1769



TÍTULO: Effect of a recent parenteral dexamethasone and ketoprofen administration on the immunological diagnosis of tuberculosis in goats


REVISTA: Front Vet Sci


NUMERACIÓN: 1-13


AÑO: 2022


EDITORIAL: Lausanne : Frontiers Media S.A


AUTORES: Ortega J., de Juan L., Sevilla IA., Garrido JM., Velasco C., Romero B., Dominguez M., Perez del Val B., Nebot C., Saez-Llorente JL., Alvarez J. and Bezos J..


PARTICIPANTES VISAVET


First
Javier Ortega Martín
2nd
Lucía de Juan Ferré
6th
Beatriz Romero Martínez
11th
Julio Álvarez Sánchez
Last
Javier Bezos Garrido

DOI: https://doi.org/10.3389/fvets.2022.1042428


CITA ESTA PUBLICACIÓN:

Ortega J., de Juan L., Sevilla IA., Garrido JM., Velasco C., Romero B., Dominguez M., Perez del Val B., Nebot C., Saez-Llorente JL., Alvarez J. y Bezos J. Effect of a recent parenteral dexamethasone and ketoprofen administration on the immunological diagnosis of tuberculosis in goats. Frontiers in veterinary science. 1-13. 2022. (A). ISSN: 2297-1769. DOI: 10.3389/fvets.2022.1042428


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