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Effect of paratuberculosis on the diagnosis of bovine tuberculosis in a cattle herd with a mixed infection using interferon-gamma detection assay

Veterinary Microbiology publica este artículo de investigación

30 de marzo de 2009

Interferon-gamma (IFN-gamma) detection assay is being applied as an ancillary test to tuberculin tests in the diagnosis of bovine tuberculosis to detect the maximum number of infected animals. Among possible factors influencing the performance of tuberculosis-diagnostic tests, paratuberculosis, a widespread disease in Spain and other European countries, has been pointed out as a cause of false positive reactions. Still, its effect on the sensitivity of these tests in cattle has yet to be fully characterized. The impact of paratuberculosis in the apparent sensitivity of IFN-gamma assay was studied in a bullfighting cattle herd with a mixed tuberculosis-paratuberculosis infection, using culture of Mycobacterium bovis and Mycobacterium avium paratuberculosis as the gold standard to determine the infection status of every animal. A total of 218 animals were slaughtered and sampled for bacteriology after blood sampling. IFN-gamma assay showed a lower apparent sensitivity in animals with a mixed infection (50%) compared to all animals suffering tuberculosis (78.3%). This finding indicates that the presence of paratuberculosis in tuberculosis-infected herds could imply a serious impairment in the sensitivity of IFN-gamma detection test




Alvarez J., de Juan L., Bezos J., Romero B., Saez-Llorente JL., Marques S., Dominguez C., Minguez O., Fernandez-Mardomingo B., Mateos A., Dominguez L. y Aranaz A.




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Effect of paratuberculosis on the diagnosis of bovine tuberculosis in a cattle herd with a mixed infection using interferon-gamma detection assay

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Effect of paratuberculosis on the diagnosis of bovine tuberculosis in a cattle herd with a mixed infection using interferon-gamma detection assay



Participantes:

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Ministerio de Agricultura, Pesca y AlimentaciónDirección General de Ganaderia. Ministerio de Agricultura, Pesca y Alimentación (MAPA).

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Junta de Castilla y LeónServicio de Sanidad Animal. Junta de Castilla y León.

Junta de Castilla y LeónLaboratorio Regional de Sanidad Animal. Junta de Castilla y León.







Veterinary Microbiology
FACTOR YEAR Q
2.874 2009

NLMID: 7705469

PMID: 18986776

ISSN: 0378-1135



TÍTULO: Effect of paratuberculosis on the diagnosis of bovine tuberculosis in a cattle herd with a mixed infection using interferon-gamma detection assay


REVISTA: Vet. Microbiol.


NUMERACIÓN: 135:389-393


AÑO: 2009


EDITORIAL: Elsevier Science Publishers


AUTORES: Alvarez J., de Juan L., Bezos J., Romero B., Saez-Llorente JL., Marques S., Dominguez C., Minguez O., Fernandez-Mardomingo B., Mateos A., Dominguez L. and Aranaz A.


PARTICIPANTES VISAVET


First
Julio Álvarez Sánchez
2nd
Lucía de Juan Ferré
3rd
Javier Bezos Garrido
4th
Beatriz Romero Martínez
11th
Lucas Domínguez Rodríguez

DOI: https://doi.org/10.1016/j.vetmic.2008.09.060


CITA ESTA PUBLICACIÓN:

Alvarez J., de Juan L., Bezos J., Romero B., Saez-Llorente JL., Marques S., Dominguez C., Minguez O., Fernandez-Mardomingo B., Mateos A., Dominguez L. y Aranaz A. Effect of paratuberculosis on the diagnosis of bovine tuberculosis in a cattle herd with a mixed infection using interferon-gamma detection assay. Veterinary Microbiology. 135:389-393. 2009. (A). ISSN: 0378-1135. DOI: 10.1016/j.vetmic.2008.09.060


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